caa a22 4500 467928835 CHVBK 20180406152937.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s10658-006-9055-y doi (NATIONALLICENCE)springer-10.1007/s10658-006-9055-y Eucalyptusoccidentalis plantlets are naturally infected by pathogenic Agrobacteriumtumefaciens [Elektronische Daten] [Zoulikha Krimi, Aïda Raio, Annik Petit, Xavier Nesme, Yves Dessaux] In the Sidi M'djahed nursery (Algeria), over 60,000 eucalyptus (Eucalyptusoccidentalis) plantlets exhibited tumour-like growths localized at the crown of the plants that resembled crown galls caused by Agrobacteriumtumefaciens. Bacteria colonizing the galls were isolated and purified. Most (22 out of 24) of the isolates had cultural and biochemical characteristics similar to those of strains of the biovar 1 of A.tumefaciens. Twenty out of 22 Agrobacterium isolates induced tumour formation on various test plants. In PCR experiments, DNA extracted from these virulent strains yielded an amplification signal corresponding to a 247-bp fragment located within the virulence region of nopaline type Ti plasmid. Consistent with this, the opine nopaline was detected in the tumours induced on test plants - but not on eucalyptus plants. Nopaline was degraded by the 20 pathogenic isolates that were also sensitive to agrocin 84, indicating the presence of a nopaline-type pTi in these strains. The chromosomal region encoding the 16S rRNA was analyzed in a sub-population of the pathogenic agrobacterial isolates. The analyzed strains were found to belong to the ribogroup of the reference strain B6. Interestingly, Eucalyptuscamaldulensis and Eucalyptuscladocalyx grown in the same nursery and in the same soil substrate developed no galls. Springer Science + Business Media B.V., 2006 16S rRNA nationallicence agrocin 84 nationallicence opine nationallicence plant pathogen nationallicence Ti plasmid nationallicence ITS : Intergenic transcribed spacer nationallicence MG : Mannitol glutamate nationallicence MS : Murashige and Skoog nationallicence tms : tumour morphology shoot nationallicence vir : virulence nationallicence Krimi Zoulikha Laboratoire de Phytobactériologie, Faculté des Sciences Agronomiques et Vétérinaires, Université de Blida, 09000, Blida, Algérie aut Raio Aïda Instituto per la Protezione delle Piante - CNR, Via Università, 133, 80055, Portici, Napoli, Italy aut Petit Annik Institut des Sciences du Végétal, UPR 2355 CNRS, 91198, Gif-sur-Yvette Cedex, France aut Nesme Xavier UMR CNRS 5557 Ecologie Microbienne and USC INRA 1193, IFR41 Bio-Environnement et Santé, UniversitéLyon1, 69622, Villeurbanne Cedex, France aut Dessaux Yves Institut des Sciences du Végétal, UPR 2355 CNRS, 91198, Gif-sur-Yvette Cedex, France aut European Journal of Plant Pathology Kluwer Academic Publishers 116/3(2006-11-01), 237-246 0929-1873 116:3<237 2006 116 10658 https://doi.org/10.1007/s10658-006-9055-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-006-9055-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Krimi Zoulikha Laboratoire de Phytobactériologie, Faculté des Sciences Agronomiques et Vétérinaires, Université de Blida, 09000, Blida, Algérie aut NATIONALLICENCE 700 1- Raio Aïda Instituto per la Protezione delle Piante - CNR, Via Università, 133, 80055, Portici, Napoli, Italy aut NATIONALLICENCE 700 1- Petit Annik Institut des Sciences du Végétal, UPR 2355 CNRS, 91198, Gif-sur-Yvette Cedex, France aut NATIONALLICENCE 700 1- Nesme Xavier UMR CNRS 5557 Ecologie Microbienne and USC INRA 1193, IFR41 Bio-Environnement et Santé, UniversitéLyon1, 69622, Villeurbanne Cedex, France aut NATIONALLICENCE 700 1- Dessaux Yves Institut des Sciences du Végétal, UPR 2355 CNRS, 91198, Gif-sur-Yvette Cedex, France aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 116/3(2006-11-01), 237-246 0929-1873 116:3<237 2006 116 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer