caa a22 4500 467928878 CHVBK 20180406152937.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s10658-006-9061-0 doi (NATIONALLICENCE)springer-10.1007/s10658-006-9061-0 Genetic variability and virulence among Verticillium albo-atrum isolates from hop [Elektronische Daten] [Sebastjan Radišek, Jernej Jakše, Branka Javornik] Verticillium wilt, caused by Verticillium albo-atrum or V. dahliae, is an important disease of many worldwide crop species. In Europe, V. albo-atrum isolates infecting hop express different levels of virulence, inducing mild or lethal disease syndromes, and it is therefore an attractive model for studying the virulence of this pathogen. In this work, eleven amplified fragment length polymorphism (AFLP) primer combinations were used to analyze genetic variability among 55 V. albo-atrum hop isolates from four European hop growing regions, as well as isolates from other hosts and V. dahliae isolates. Cluster analysis divided V. albo-atrum and V. dahliae isolates into two well-separated groups. Within the V. dahliae cluster, isolates were separated without host specific grouping, although no host adapted isolates were included. In V. albo-atrum, the alfalfa isolates were distinct from isolates of other hosts, where a high association with virulence was observed in hop and tomato isolates. All lethal hop isolates were genetically different from mild hop isolates. The lethal hop isolates from England and Slovenia expressed the same virulence phenotype, although they showed a different AFLP pattern. The mild hop isolates formed two subgroups, to which isolates clustered irrespective of geographical location. These data suggest multiple origins of V. albo-atrum hop isolates, and the possible appearance of new virulent isolates in the future in other hop growing regions. Springer Science + Business Media B.V., 2006 Humulus lupulus nationallicence molecular markers nationallicence Verticillium wilt nationallicence Radišek Sebastjan Plant Protection Department, Slovenian Institute for Hop Research and Brewing, Cesta Žalskega tabora 2, SI-3310, Žalec, Slovenia aut Jakše Jernej University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1000, Ljubljana, Slovenia aut Javornik Branka University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1000, Ljubljana, Slovenia aut European Journal of Plant Pathology Kluwer Academic Publishers 116/4(2006-12-01), 301-314 0929-1873 116:4<301 2006 116 10658 https://doi.org/10.1007/s10658-006-9061-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-006-9061-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Radišek Sebastjan Plant Protection Department, Slovenian Institute for Hop Research and Brewing, Cesta Žalskega tabora 2, SI-3310, Žalec, Slovenia aut NATIONALLICENCE 700 1- Jakše Jernej University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1000, Ljubljana, Slovenia aut NATIONALLICENCE 700 1- Javornik Branka University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1000, Ljubljana, Slovenia aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 116/4(2006-12-01), 301-314 0929-1873 116:4<301 2006 116 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer