caa a22 4500 467928894 CHVBK 20180406152937.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s10658-006-9060-1 doi (NATIONALLICENCE)springer-10.1007/s10658-006-9060-1 Improved method for assaying maize plant resistance to maize rough dwarf disease by artificial inoculation and real-time RT-PCR [Elektronische Daten] [Fei Wang, Guozheng Qin, Zhenhua Sui, Zhaohui Wang, Zhaoyu Wang, Jialin Yu, Juren Zhang] The study of maize rough dwarf disease (MRDD) and breeding for resistance requires inoculation of maize plants by means of planthoppers. The plant age, insect density and inoculation duration are main factors in the success of maize rough dwarf disease inoculation. These parameters were tested using a susceptible maize inbred line Ye478. Using one or two-leaf plants, 15 planthoppers per plant and a five day inoculation duration, the line Ye478 was the most susceptible with 100% diseased plants; F112132 was moderately susceptible with 60% diseased plants and 90110 and F022411 were resistant without any disease. The results were consistent with those from sixyears of field studies. Using enzyme-linked immunosorbent assay (ELISA) and real-time quantitative RT-PCR, rice black-streaked dwarf virus was detected in severely diseased plants. The plants were rated from 0 to 3 according to their symptoms at the time of flowering. Plants scoring 0, 1 and 2 could not be distinguished by ELISA, only by real-time quantitative RT-PCR. All of the plants with a score of 3 were positive by ELISA and real-time quantitative RT-PCR. The significant differences in the average viral contents in plants with different symptom ratings could be distinguished by using real-time RT-PCR. Springer Science + Business Media B.V., 2006 maize nationallicence maize rough dwarf disease nationallicence real-time RT-PCR nationallicence rice black-streaked dwarf virus nationallicence Wang Fei School of Life Science, Shandong University, 250100, Jinan, P.R. China aut Qin Guozheng School of Life Science, Shandong University, 250100, Jinan, P.R. China aut Sui Zhenhua School of Life Science, Shandong University, 250100, Jinan, P.R. China aut Wang Zhaohui State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University, 100094, Beijing, P.R. China aut Wang Zhaoyu School of Life Science, Shandong University, 250100, Jinan, P.R. China aut Yu Jialin State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University, 100094, Beijing, P.R. China aut Zhang Juren School of Life Science, Shandong University, 250100, Jinan, P.R. China aut European Journal of Plant Pathology Kluwer Academic Publishers 116/4(2006-12-01), 289-300 0929-1873 116:4<289 2006 116 10658 https://doi.org/10.1007/s10658-006-9060-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-006-9060-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Fei School of Life Science, Shandong University, 250100, Jinan, P.R. China aut NATIONALLICENCE 700 1- Qin Guozheng School of Life Science, Shandong University, 250100, Jinan, P.R. China aut NATIONALLICENCE 700 1- Sui Zhenhua School of Life Science, Shandong University, 250100, Jinan, P.R. China aut NATIONALLICENCE 700 1- Wang Zhaohui State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University, 100094, Beijing, P.R. China aut NATIONALLICENCE 700 1- Wang Zhaoyu School of Life Science, Shandong University, 250100, Jinan, P.R. China aut NATIONALLICENCE 700 1- Yu Jialin State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University, 100094, Beijing, P.R. China aut NATIONALLICENCE 700 1- Zhang Juren School of Life Science, Shandong University, 250100, Jinan, P.R. China aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 116/4(2006-12-01), 289-300 0929-1873 116:4<289 2006 116 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer