caa a22 4500 467928916 CHVBK 20180406152937.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s10658-006-9057-9 doi (NATIONALLICENCE)springer-10.1007/s10658-006-9057-9 Chemical and biological protection of grapevine propagation material from trunk disease pathogens [Elektronische Daten] [P. Fourie, F. Halleen] Wound protection during all stages of grapevine propagation is of utmost importance to prevent infection of propagation material by decline and dieback pathogens. In semi-commercial nursery trials, grapevine rootstock and scion cuttings were soaked in water (control), chemical or biological sanitation products prior to cold storage, prior to grafting (machine- or hand-grafting) and prior to planting in field nurseries. Natural infection levels in basal ends and graft unions of uprooted nursery grapevines were evaluated 8months after planting. Total pathogen incidences in the water-treated control plants ranged from 30% in basal ends to 13.5% in graft unions. Phaeomoniella chlamydospora was the most commonly isolated pathogen, followed by Phaeoacremonium, Cylindrocarpon+Campylocarpon, Botryosphaeria and Phomopsis spp. Machine-grafted unions generally had lower pathogen incidences compared with hand-grafted graft unions. In general, repeated soak-treatments of propagation material in the tested products resulted in reduced pathogen incidences in nursery grapevines. However, products containing T. harzianum (Trichoflow-T), hydrogen peroxide (Bio-sterilizer) and 8-hydroxyquinoline sulphate (Chinosol) gave inconsistent results, whereas Bronocide (a blend of halogenated alcohols and water) proved to be a good sterilising agent, but reduced certifiable plant yield significantly. Benomyl (at 100g/100l), Sporekill (a patented didecyldimethylammonium chloride formulation at 150ml/100l) and captan (at 1000ml/100l) were consistently the best treatments as growth parameters were not negatively influenced and pathogen incidences in basal ends and graft unions of uprooted plants were reduced. Springer Science + Business Media B.V., 2006 benomyl nationallicence captan nationallicence nursery nationallicence Phaeoacremonium nationallicence Phaeomoniella nationallicence Sporekill nationallicence Fourie P. Department of Plant Pathology, University of Stellenbosch, Private Bag X1, 7602, Stellenbosch, South Africa aut Halleen F. Plant Protection Division, ARC Infruitec-Nietvoorbij, Private Bag X5026, 7599, Stellenbosch, South Africa aut European Journal of Plant Pathology Kluwer Academic Publishers 116/4(2006-12-01), 255-265 0929-1873 116:4<255 2006 116 10658 https://doi.org/10.1007/s10658-006-9057-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-006-9057-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fourie P. Department of Plant Pathology, University of Stellenbosch, Private Bag X1, 7602, Stellenbosch, South Africa aut NATIONALLICENCE 700 1- Halleen F. Plant Protection Division, ARC Infruitec-Nietvoorbij, Private Bag X5026, 7599, Stellenbosch, South Africa aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 116/4(2006-12-01), 255-265 0929-1873 116:4<255 2006 116 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer