caa a22 4500 467928940 CHVBK 20180406152937.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s10658-006-9042-3 doi (NATIONALLICENCE)springer-10.1007/s10658-006-9042-3 Viral reinfection affecting bulb production in garlic after seven years of cultivation under field conditions [Elektronische Daten] [Péricles Filho, Renato Resende, Célia Cordeiro, José Buso, Antonio Torres, André Dusi] Six sequential field experiments were conducted from 1999 to 2002 to evaluate virus reinfection in garlic cv. Amarante and its effect on bulb production. The treatments in the year 1999 were: T1 - virus-free garlic-seed obtained by thermotherapy and meristem-tip culture and indexed for virus by immuno-sorbent electron microscopy (ISEM) under its first field cycle; T2, T3 and T4 - garlic-seed in the second, third and fourth field cycles, respectively; and C - standard garlic-seed from the grower (with no control of virus infection) as a control. In the years 2000 to 2002, a new plot of virus-free seed was added to the experiment and cloves from the previous treatments were again grown under field conditions. In the fourth year of experiments, the treatments comprised T1 (virus-free garlic seeds under the first field cycle) to T7 (garlic-seed in the sixth field cycle) and C. Two experiments were conducted in the years 1999 and 2000, at two locations and in the years 2001 and 2002 only one experiment per year at one location. The combined analysis of variance for all experiments indicated a significant difference between the treatments for plant height and yield. The bulbs were classified into commercial classes according the Brazilian regulation and the commercial classes 4-7 were 72 %, 60%, 59%, 53% and 35% of the total number of bulbs harvested, from T1 to T5, respectively. Virus reinfection at the end of the second field cycle, determined by serology using antisera against the most common viruses of garlic in Brazil reached 83%. Treatments T1-T7 yielded 118%, 79%, 57%, 51%, 39%, 33% and 31% higher than the control. Koninklijke Nederlandse Planteziektenkundige Vereniging (KNPV), 2006 epidemiology nationallicence garlic-seed nationallicence potyvirus nationallicence spatial-spread nationallicence temporal-spread nationallicence virus-free plants nationallicence Filho Péricles Departamento de Agronomia, Universidade Federal Rural de Pernambuco, CEP 52.171-900, Recife, PE, Brasil aut Resende Renato Departamento de Biologia Celular, Universidade de Brasília, CEP 70919-900, Brasília, DF, Brasil aut Cordeiro Célia Embrapa Recursos Genéticos e Biotecnologia, Caixa Postal 02372, 70770-900, Brasília, DF, Brasil aut Buso José Embrapa Hortaliças, Caixa Postal 218, CEP 70359-970, Brasília, DF, Brasil aut Torres Antonio Embrapa Hortaliças, Caixa Postal 218, CEP 70359-970, Brasília, DF, Brasil aut Dusi André Embrapa Hortaliças, Caixa Postal 218, CEP 70359-970, Brasília, DF, Brasil aut European Journal of Plant Pathology Kluwer Academic Publishers 116/2(2006-10-01), 95-101 0929-1873 116:2<95 2006 116 10658 https://doi.org/10.1007/s10658-006-9042-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-006-9042-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Filho Péricles Departamento de Agronomia, Universidade Federal Rural de Pernambuco, CEP 52.171-900, Recife, PE, Brasil aut NATIONALLICENCE 700 1- Resende Renato Departamento de Biologia Celular, Universidade de Brasília, CEP 70919-900, Brasília, DF, Brasil aut NATIONALLICENCE 700 1- Cordeiro Célia Embrapa Recursos Genéticos e Biotecnologia, Caixa Postal 02372, 70770-900, Brasília, DF, Brasil aut NATIONALLICENCE 700 1- Buso José Embrapa Hortaliças, Caixa Postal 218, CEP 70359-970, Brasília, DF, Brasil aut NATIONALLICENCE 700 1- Torres Antonio Embrapa Hortaliças, Caixa Postal 218, CEP 70359-970, Brasília, DF, Brasil aut NATIONALLICENCE 700 1- Dusi André Embrapa Hortaliças, Caixa Postal 218, CEP 70359-970, Brasília, DF, Brasil aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 116/2(2006-10-01), 95-101 0929-1873 116:2<95 2006 116 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer