caa a22 4500 467928967 CHVBK 20180406152937.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s10658-006-9045-0 doi (NATIONALLICENCE)springer-10.1007/s10658-006-9045-0 Two subpopulations of Colletotrichum acutatum are responsible for anthracnose in strawberry and leatherleaf fern in Costa Rica [Elektronische Daten] [Michaela Schiller, Mette Lübeck, Thomas Sundelin, Luis Meléndez, Solveig Danielsen, Dan Jensen, Kenneth Ordeñana] Strawberry, Fragaria × ananassa, and leatherleaf fern, Rumohra adiantiformis, are two important crops in Costa Rica. One of the most severe diseases affecting these crops is anthracnose, caused by members of the fungal genus, Colletotrichum (teleomorph; Glomerella). Eighty single-spore isolates from strawberry and leatherleaf fern were identified as Colletotrichum acutatum by species-specific PCR, and were further characterised by Universally Primed PCR (UP-PCR) fingerprinting analysis, and sequence analysis of the ribosomal internal transcribed spacer (ITS) region. Morphological differences, genotypic variation revealed by UP-PCR fingerprinting analysis, and a single sequence polymorphism within the ITS2 region were found between the isolates from strawberry and leatherleaf fern, respectively. The UPGMA cluster analysis of the fingerprints clearly separated the isolates derived from strawberry and leatherleaf fern into two different clusters. Pathogenicity assays on detached strawberry fruits confirmed the apparent difference between the two groups of isolates. It is therefore suggested that the pathogens responsible for strawberry anthracnose fruit rot and leatherleaf fern anthracnose in Costa Rica, belong to two distinct subpopulations of C. acutatum. Springer Science + Business Media B.V., 2006 anthracnose nationallicence Fragaria × ananassa nationallicence internal transcribed spacer (ITS) region nationallicence pathogenicity test nationallicence Rumohra adiantiformis nationallicence Universally Primed polymerase chain reaction (UP-PCR) nationallicence ITS : internal transcribed spacer nationallicence rDNA : ribosomal DNA nationallicence UPGMA : unweighted pair-group method using arithmetic average nationallicence UP-PCR : Universally primed polymerase chain reaction nationallicence Schiller Michaela Department of Plant Biology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, Frederiksberg C, 1871, Copenhagen, Denmark aut Lübeck Mette Department of Plant Biology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, Frederiksberg C, 1871, Copenhagen, Denmark aut Sundelin Thomas Department of Plant Biology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, Frederiksberg C, 1871, Copenhagen, Denmark aut Meléndez Luis Research Centre of Crop Protection (CIPROC), Faculty of Agronomy and Food Science, University of Costa Rica, BioTécnica Análisis Moleculares S.A., San Pedro, San José, Costa Rica aut Danielsen Solveig Department of Plant Biology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, Frederiksberg C, 1871, Copenhagen, Denmark aut Jensen Dan Department of Plant Biology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, Frederiksberg C, 1871, Copenhagen, Denmark aut Ordeñana Kenneth Research Centre of Crop Protection (CIPROC), Faculty of Agronomy and Food Science, University of Costa Rica, BioTécnica Análisis Moleculares S.A., San Pedro, San José, Costa Rica aut European Journal of Plant Pathology Kluwer Academic Publishers 116/2(2006-10-01), 107-118 0929-1873 116:2<107 2006 116 10658 https://doi.org/10.1007/s10658-006-9045-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-006-9045-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schiller Michaela Department of Plant Biology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, Frederiksberg C, 1871, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Lübeck Mette Department of Plant Biology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, Frederiksberg C, 1871, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Sundelin Thomas Department of Plant Biology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, Frederiksberg C, 1871, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Meléndez Luis Research Centre of Crop Protection (CIPROC), Faculty of Agronomy and Food Science, University of Costa Rica, BioTécnica Análisis Moleculares S.A., San Pedro, San José, Costa Rica aut NATIONALLICENCE 700 1- Danielsen Solveig Department of Plant Biology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, Frederiksberg C, 1871, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Jensen Dan Department of Plant Biology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, Frederiksberg C, 1871, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Ordeñana Kenneth Research Centre of Crop Protection (CIPROC), Faculty of Agronomy and Food Science, University of Costa Rica, BioTécnica Análisis Moleculares S.A., San Pedro, San José, Costa Rica aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 116/2(2006-10-01), 107-118 0929-1873 116:2<107 2006 116 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer