caa a22 4500 467929017 CHVBK 20180406152938.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s10658-006-9051-2 doi (NATIONALLICENCE)springer-10.1007/s10658-006-9051-2 Usefulness of single copy genes containing introns in Phytophthora for the development of detection tools for the regulated species P. ramorum and P. fragariae [Elektronische Daten] [Renaud Ioos, Lise Laugustin, Nathalie Schenck, Sylvie Rose, Claude Husson, Pascal Frey] Introns are generally highly polymorphic regions within genes and were proven to be of great interest for discriminating among phylogenetically-close Phytophthora species. Phytophthora ramorum and P. fragariae are considered as quarantine pathogens by the European Union and accurate detection tools are therefore necessary for their monitoring. From introns located in different single copy genes (GPA1, RAS-like, and TRP1), we developed a series of PCR primers specific to P. ramorum and P. fragariae. The specificity of these primers was successfully checked with a wide collection of Phytophthora isolates and a protocol was developed to detect both pathogens directly in infected plant tissues. These genes should be of particular interest for the development of additional species-specific detection tools within the Phytophthora genus. Springer Science + Business Media B.V., 2006 confirmation test nationallicence degenerate primers nationallicence diagnosis nationallicence PCR nationallicence Ioos Renaud Laboratoire National de la Protection des Végétaux, Unité de Mycologie Agricole et Forestière (LNPV-UMAF), Domaine de Pixérécourt, 54220, Malzéville, France aut Laugustin Lise Laboratoire National de la Protection des Végétaux, Unité de Mycologie Agricole et Forestière (LNPV-UMAF), Domaine de Pixérécourt, 54220, Malzéville, France aut Schenck Nathalie Laboratoire National de la Protection des Végétaux, Unité de Mycologie Agricole et Forestière (LNPV-UMAF), Domaine de Pixérécourt, 54220, Malzéville, France aut Rose Sylvie Laboratoire National de la Protection des Végétaux, Unité de Mycologie Agricole et Forestière (LNPV-UMAF), Domaine de Pixérécourt, 54220, Malzéville, France aut Husson Claude INRA, UMR IAM, Equipe de Pathologie Forestière, 54280, Champenoux, France aut Frey Pascal INRA, UMR IAM, Equipe de Pathologie Forestière, 54280, Champenoux, France aut European Journal of Plant Pathology Kluwer Academic Publishers 116/2(2006-10-01), 171-176 0929-1873 116:2<171 2006 116 10658 https://doi.org/10.1007/s10658-006-9051-2 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-006-9051-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ioos Renaud Laboratoire National de la Protection des Végétaux, Unité de Mycologie Agricole et Forestière (LNPV-UMAF), Domaine de Pixérécourt, 54220, Malzéville, France aut NATIONALLICENCE 700 1- Laugustin Lise Laboratoire National de la Protection des Végétaux, Unité de Mycologie Agricole et Forestière (LNPV-UMAF), Domaine de Pixérécourt, 54220, Malzéville, France aut NATIONALLICENCE 700 1- Schenck Nathalie Laboratoire National de la Protection des Végétaux, Unité de Mycologie Agricole et Forestière (LNPV-UMAF), Domaine de Pixérécourt, 54220, Malzéville, France aut NATIONALLICENCE 700 1- Rose Sylvie Laboratoire National de la Protection des Végétaux, Unité de Mycologie Agricole et Forestière (LNPV-UMAF), Domaine de Pixérécourt, 54220, Malzéville, France aut NATIONALLICENCE 700 1- Husson Claude INRA, UMR IAM, Equipe de Pathologie Forestière, 54280, Champenoux, France aut NATIONALLICENCE 700 1- Frey Pascal INRA, UMR IAM, Equipe de Pathologie Forestière, 54280, Champenoux, France aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 116/2(2006-10-01), 171-176 0929-1873 116:2<171 2006 116 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer