caa a22 4500 467929068 CHVBK 20180406152938.0 cr unu---uuuuu 170328e20060101xx s 000 0 eng 10.1007/s10658-005-3168-6 doi (NATIONALLICENCE)springer-10.1007/s10658-005-3168-6 Genetic Linkage Maps and Genomic Organization in Leptosphaeria maculans [Elektronische Daten] [Marie-Line Kuhn, Lilian Gout, Barbara Howlett, Delphine Melayah, Michel Meyer, Marie-Hélène Balesdent, Thierry Rouxel] Leptosphaeria maculans is a haploid outcrossing ascomycete with a genome size of about 34Megabases (Mb) which is predicted to have between 10,000 and 12,000 genes. The chromosomes of L. maculans are of a size range (0.7-3.5Mb) and number (15-16) that can be readily resolved by pulsed field gel electrophoresis. Chromosome length polymorphisms are generated by meiosis giving rise to size differences as high as 57%, in the case of the ribosomal DNA-harbouring chromosome whose size varies between 1.8 and 4.2Mb. Genetic maps are characterised by linkage groups comprising an accumulation of markers based on retrotransposon sequences. This, along with sequencing of pericentromeric regions and stretches of ORF-rich regions, suggest that the genome of L. maculans consists of a mosaic of GC-equilibrated coding regions with no or few transposons, and of stretches of highly degenerated and truncated retrotransposons, encompassing very few genes. Chromosome length polymorphisms are linked with the loss of dispensable regions. We suggest that the degree of length polymorphism for a particular chromosome correlates to the amount of dispensable retrotransposons, and that some gene-rich chromosomes may be less prone to undergo chromosome length polymorphisms than other chromosomes. Springer, 2006 chromosome length polymorphism nationallicence genetic map nationallicence genome structure nationallicence physical map nationallicence repetitive DNA nationallicence retrotransposons nationallicence AFLP : amplified fragment length polymorphism nationallicence BAC : bacterial artificial chromosome nationallicence CHEF : contour-clamped homogeneous electric field nationallicence CLP : chromosome length polymorphism nationallicence ISSR : inter-simple sequence repeat nationallicence LG : linkage group nationallicence LTR : long terminal repeats nationallicence RAPD : random amplified polymorphic DNA nationallicence Rep-PCR : repetitive element-based PCR nationallicence RIP : repeat induced point mutation nationallicence TAFE : transverse alternating field electrophoresis nationallicence Kuhn Marie-Line INRA-PMDV, Route de St Cyr, 78026, Versailles Cedex, France aut Gout Lilian INRA-PMDV, Route de St Cyr, 78026, Versailles Cedex, France aut Howlett Barbara School of Botany, The University of Melbourne, 3010, Victoria, Australia aut Melayah Delphine INRA-PMDV, Route de St Cyr, 78026, Versailles Cedex, France aut Meyer Michel INRA-PMDV, Route de St Cyr, 78026, Versailles Cedex, France aut Balesdent Marie-Hélène INRA-PMDV, Route de St Cyr, 78026, Versailles Cedex, France aut Rouxel Thierry INRA-PMDV, Route de St Cyr, 78026, Versailles Cedex, France aut European Journal of Plant Pathology Kluwer Academic Publishers 114/1(2006-01-01), 17-31 0929-1873 114:1<17 2006 114 10658 https://doi.org/10.1007/s10658-005-3168-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-005-3168-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kuhn Marie-Line INRA-PMDV, Route de St Cyr, 78026, Versailles Cedex, France aut NATIONALLICENCE 700 1- Gout Lilian INRA-PMDV, Route de St Cyr, 78026, Versailles Cedex, France aut NATIONALLICENCE 700 1- Howlett Barbara School of Botany, The University of Melbourne, 3010, Victoria, Australia aut NATIONALLICENCE 700 1- Melayah Delphine INRA-PMDV, Route de St Cyr, 78026, Versailles Cedex, France aut NATIONALLICENCE 700 1- Meyer Michel INRA-PMDV, Route de St Cyr, 78026, Versailles Cedex, France aut NATIONALLICENCE 700 1- Balesdent Marie-Hélène INRA-PMDV, Route de St Cyr, 78026, Versailles Cedex, France aut NATIONALLICENCE 700 1- Rouxel Thierry INRA-PMDV, Route de St Cyr, 78026, Versailles Cedex, France aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 114/1(2006-01-01), 17-31 0929-1873 114:1<17 2006 114 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer