caa a22 4500 467929165 CHVBK 20180406152938.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s10658-005-5995-x doi (NATIONALLICENCE)springer-10.1007/s10658-005-5995-x Assessment of Partial Resistance to Powdery Mildew ( Podosphaera pannosa ) in a Tetraploid Rose Population Using a Spore-suspension Inoculation Method [Elektronische Daten] [Zifu Yan, Oene Dolstra, Theo Prins, Piet Stam, Peter Visser] Powdery mildew (Podosphaera pannosa) is the most important fungal disease in greenhouse roses and is in practice controlled by fungicides. The creation of novel cultivars with durable resistance to powdery mildew is highly desirable. To understand the inheritance of mildew resistance, a tetraploid rose population with a size of 181 seedlings was obtained by crossing two tetraploid genotypes each having partial resistance. The population and its parents were tested under greenhouse conditions with two well-defined monospore isolates (2 and F1) using artificial inoculation with spore suspensions. Disease score at 11days post-inoculation, latent period and rate of symptom development were used to describe seedling resistance. The tests for both isolates exhibited a wide and significant variation among genotypes for resistance. The distribution of the genotypic means of the disease scores was continuous and showed a considerable transgression. Statistical analysis, scatter plot of disease scores for the isolates, and correlation analyses indicated that the two isolates differed in pathogenicity. The outcome of the tests showed that the inoculation assay with spore suspensions was a reliable and effective way to screen large numbers of genotypes under greenhouse conditions for genetic and breeding studies. This is the first report on spore-suspension inoculation to be used successfully in rose. Springer, 2006 durable resistance nationallicence greenhouse test nationallicence latent period nationallicence monospore isolate nationallicence Rosa nationallicence Yan Zifu Plant Research International B.V., Wageningen University and Research Centre, P.O. Box 16, 6700, Wageningen, AA, The Netherlands aut Dolstra Oene Plant Research International B.V., Wageningen University and Research Centre, P.O. Box 16, 6700, Wageningen, AA, The Netherlands aut Prins Theo Plant Research International B.V., Wageningen University and Research Centre, P.O. Box 16, 6700, Wageningen, AA, The Netherlands aut Stam Piet Laboratory of Plant Breeding, Wageningen University and Research Centre, P.O. Box 386, 6700, Wageningen, AJ, The Netherlands aut Visser Peter Plant Research International B.V., Wageningen University and Research Centre, P.O. Box 16, 6700, Wageningen, AA, The Netherlands aut European Journal of Plant Pathology Kluwer Academic Publishers 114/3(2006-03-01), 301-308 0929-1873 114:3<301 2006 114 10658 https://doi.org/10.1007/s10658-005-5995-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-005-5995-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yan Zifu Plant Research International B.V., Wageningen University and Research Centre, P.O. Box 16, 6700, Wageningen, AA, The Netherlands aut NATIONALLICENCE 700 1- Dolstra Oene Plant Research International B.V., Wageningen University and Research Centre, P.O. Box 16, 6700, Wageningen, AA, The Netherlands aut NATIONALLICENCE 700 1- Prins Theo Plant Research International B.V., Wageningen University and Research Centre, P.O. Box 16, 6700, Wageningen, AA, The Netherlands aut NATIONALLICENCE 700 1- Stam Piet Laboratory of Plant Breeding, Wageningen University and Research Centre, P.O. Box 386, 6700, Wageningen, AJ, The Netherlands aut NATIONALLICENCE 700 1- Visser Peter Plant Research International B.V., Wageningen University and Research Centre, P.O. Box 16, 6700, Wageningen, AA, The Netherlands aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 114/3(2006-03-01), 301-308 0929-1873 114:3<301 2006 114 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer