caa a22 4500 467929173 CHVBK 20180406152938.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s10658-005-5997-8 doi (NATIONALLICENCE)springer-10.1007/s10658-005-5997-8 Microscopic, Biochemical and Physiological Assessment of the Effect of Methylene Bisthiocyanate on the Sapstain Fungus Ophiostoma floccosum [Elektronische Daten] [Tripti Singh, Bernhard Kreber, Adya Singh, Alison Stewart, Marlene Jaspers] In vitro effects of methylene bisthiocyanate (MBT) on hyphal morphology and ultra-structure of Ophiostoma floccosum were examined using differential interference contrast, epifluorescence and transmission electron microscopy (TEM). To understand the mode of action of MBT, experiments were undertaken to measure potassium ion (K+) leakage from cells, oxygen consumption, glucose and ATP levels. Differential interference contrast microscopy indicated that MBT caused rapid changes in O. floccosum hyphae resulting in extensive vaculoation and accumulation of granular materials within the cytoplasm. Epifluorescence microscopy provided evidence that MBT treatment causes a loss in the permeability properties of the plasma membrane. TEM showed retraction of the plasma membrane from the cell wall, aggregation of cytoplasmic contents, vesiculation of membranous components, a dramatic increase in vacuolation, and eventually a complete loss in the integrity of organelles. There was a rapid efflux of intracellular K+ ions from cells, a substantial loss in K+ ions occurring within the first 5min of MBT treatment. The rate of K+ leakage was MBT concentration treatment-time dependent. The study also showed that the effect of lower concentrations of MBT (0.01 and 0.1mM) on respiratory activity was negligible. However, at the same concentrations, glucose consumption and ATP production were affected. Taken together, these observations suggest that the target site of MBT in O. floccosum alters membrane properties and uncouples oxidative phosphorylation from the respiratory chain. Springer, 2006 ATP nationallicence mode of action nationallicence morphology nationallicence potassium (K+) level nationallicence respiration nationallicence target site(s) nationallicence ultra-structure nationallicence Singh Tripti Ensis, Wood Processing and Products Unit, Scion, Rotorua, New Zealand aut Kreber Bernhard Ensis, Wood Processing and Products Unit, Scion, Rotorua, New Zealand aut Singh Adya Ensis, Wood Processing and Products Unit, Scion, Rotorua, New Zealand aut Stewart Alison Lincoln University, Canterbury, New Zealand aut Jaspers Marlene Lincoln University, Canterbury, New Zealand aut European Journal of Plant Pathology Kluwer Academic Publishers 114/3(2006-03-01), 317-328 0929-1873 114:3<317 2006 114 10658 https://doi.org/10.1007/s10658-005-5997-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-005-5997-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Singh Tripti Ensis, Wood Processing and Products Unit, Scion, Rotorua, New Zealand aut NATIONALLICENCE 700 1- Kreber Bernhard Ensis, Wood Processing and Products Unit, Scion, Rotorua, New Zealand aut NATIONALLICENCE 700 1- Singh Adya Ensis, Wood Processing and Products Unit, Scion, Rotorua, New Zealand aut NATIONALLICENCE 700 1- Stewart Alison Lincoln University, Canterbury, New Zealand aut NATIONALLICENCE 700 1- Jaspers Marlene Lincoln University, Canterbury, New Zealand aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 114/3(2006-03-01), 317-328 0929-1873 114:3<317 2006 114 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer