caa a22 4500 467929181 CHVBK 20180406152938.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s10658-005-5996-9 doi (NATIONALLICENCE)springer-10.1007/s10658-005-5996-9 Intraspecific Relationship of Plasmopara halstedii Isolates Differing in Pathogenicity and Geographic Origin Based on ITS Sequence Data [Elektronische Daten] [Otmar Spring, Mark Bachofer, Marco Thines, Alexandra Riethmüller, Markus Göker, Franz Oberwinkler] Sequence parts of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA were analysed to screen for the intraspecific variability of a non-coding genomic region in 15 Plasmopara halstedii populations of different pathotype and geographic origin. Samples revealed uniformity in a ca. 790 Bp fragment comprising of the ITS-1, 5.8S and front parts of the ITS-2. In contrast, clear differences were found in a ca. 810 Bp fragment of the ITS-2 thus allowing differentiation between populations of pathotype 100, 310 and 330 and a group of populations representing pathotypes 700, 701, 703, 710 and 730. Samples of pathotypes 700 to730 originated from Slovakia, France, and Germany, but were uniform in both ITS sequence parts, thus indicating very recent origin of these highly aggressive physiological races. The potential use of ITS sequences for pathotype differentiation and phylogenetic studies in P. halstedii is discussed. Springer, 2006 epidemiology nationallicence Helianthus annuus nationallicence Plasmopara helianthi nationallicence population studies nationallicence sunflower downy mildew pathotypes nationallicence Spring Otmar Institute of Botany (210), University of Hohenheim, 70593, Stuttgart, Germany aut Bachofer Mark Institute of Botany (210), University of Hohenheim, 70593, Stuttgart, Germany aut Thines Marco Institute of Botany (210), University of Hohenheim, 70593, Stuttgart, Germany aut Riethmüller Alexandra Department of Ecology, University of Kassel, Heinrich-Plett-Str. 40, 34132, Kassel, Germany aut Göker Markus Institute of Organismic Botany and Mycology, University of Tübingen, Auf der Morgenstelle 1, 72076, Tübingen, Germany aut Oberwinkler Franz Institute of Organismic Botany and Mycology, University of Tübingen, Auf der Morgenstelle 1, 72076, Tübingen, Germany aut European Journal of Plant Pathology Kluwer Academic Publishers 114/3(2006-03-01), 309-315 0929-1873 114:3<309 2006 114 10658 https://doi.org/10.1007/s10658-005-5996-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-005-5996-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Spring Otmar Institute of Botany (210), University of Hohenheim, 70593, Stuttgart, Germany aut NATIONALLICENCE 700 1- Bachofer Mark Institute of Botany (210), University of Hohenheim, 70593, Stuttgart, Germany aut NATIONALLICENCE 700 1- Thines Marco Institute of Botany (210), University of Hohenheim, 70593, Stuttgart, Germany aut NATIONALLICENCE 700 1- Riethmüller Alexandra Department of Ecology, University of Kassel, Heinrich-Plett-Str. 40, 34132, Kassel, Germany aut NATIONALLICENCE 700 1- Göker Markus Institute of Organismic Botany and Mycology, University of Tübingen, Auf der Morgenstelle 1, 72076, Tübingen, Germany aut NATIONALLICENCE 700 1- Oberwinkler Franz Institute of Organismic Botany and Mycology, University of Tübingen, Auf der Morgenstelle 1, 72076, Tübingen, Germany aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 114/3(2006-03-01), 309-315 0929-1873 114:3<309 2006 114 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer