caa a22 4500 467929300 CHVBK 20180406152938.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s10658-006-0002-8 doi (NATIONALLICENCE)springer-10.1007/s10658-006-0002-8 Molecular and Pathotype Analysis of the Rice Blast Fungus in North Vietnam [Elektronische Daten] [Nguyen Thuan, Joseph Bigirimana, Ed Roumen, Dominique Van Der Straeten, Monica Höfte] Rice blast caused by Pyricularia oryzae is a devastating disease worldwide. In Vietnam, rice blast is especially severe in the Red River Delta in the North. The genetic diversity of 114 P. oryzae isolates collected from rice in 2001 in the Red River Delta and nine additional Vietnamese P. oryzae isolates was analysed using Amplified Fragment Length Polymorphism (AFLP). DNA similarity and cluster analysis based on 160 polymorphic AFLP markers showed twelve different AFLP genetic groups among the 123 field isolates. Isolates collected from japonica hosts clustered separately from indica host isolates with at least 60% dissimilarity with little evidence for gene flow between the two populations. In the 2001 population originating from indica hosts, three genetic groups were predominant and represented 99% of the isolates sampled. One predominant clonal lineage represented 59% of the 2001 indica host population and was found in eleven provinces in the Red River Delta of North Vietnam. Significant genotype flow could be demonstrated between the indica population south of Red river and the indica population north of Red river. There was significant linkage disequilibrium between the AFLP loci within the indica population, indicating that this is not a random mating population. Pathogenicity tests of 25 isolates selected from the 12 AFLP groups on a set of 29 differential rice lines revealed two avirulent isolates and 23 pathotypes. Different combinations of known resistance genes were found to have potential for blast resistance breeding for North Vietnam. Springer, 2006 AFLP nationallicence genetic diversity nationallicence Magnaporthe grisea nationallicence Pyricularia grisea nationallicence Thuan Nguyen Laboratory of Phytopathology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000, Gent, Belgium aut Bigirimana Joseph Laboratory of Phytopathology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000, Gent, Belgium aut Roumen Ed Bayer CropScience, Technologiepark 38, B-9052, Gent, Belgium aut Van Der Straeten Dominique Department of Molecular Genetics, Ghent University, K.L. Ledeganckstraat 35, B-9000, Gent, Belgium aut Höfte Monica Laboratory of Phytopathology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000, Gent, Belgium aut European Journal of Plant Pathology Kluwer Academic Publishers 114/4(2006-04-01), 381-396 0929-1873 114:4<381 2006 114 10658 https://doi.org/10.1007/s10658-006-0002-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-006-0002-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Thuan Nguyen Laboratory of Phytopathology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000, Gent, Belgium aut NATIONALLICENCE 700 1- Bigirimana Joseph Laboratory of Phytopathology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000, Gent, Belgium aut NATIONALLICENCE 700 1- Roumen Ed Bayer CropScience, Technologiepark 38, B-9052, Gent, Belgium aut NATIONALLICENCE 700 1- Van Der Straeten Dominique Department of Molecular Genetics, Ghent University, K.L. Ledeganckstraat 35, B-9000, Gent, Belgium aut NATIONALLICENCE 700 1- Höfte Monica Laboratory of Phytopathology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000, Gent, Belgium aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 114/4(2006-04-01), 381-396 0929-1873 114:4<381 2006 114 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer