caa a22 4500 467929335 CHVBK 20180406152938.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s10658-006-0005-5 doi (NATIONALLICENCE)springer-10.1007/s10658-006-0005-5 Chitosan Stimulates Defense Reactions in Grapevine Leaves and Inhibits Development of Botrytis Cinerea [Elektronische Daten] [Patricia Trotel-Aziz, Michel Couderchet, Guy Vernet, Aziz Aziz] Chitosan (β-1,4-linked glucosamine oligomer) derived from crab shells conferred a high protection of grapevine leaves against grey mould caused by Botrytis cinerea. Under controlled conditions, it was shown to be an efficient elicitor of some defense reactions in grapevine leaves and to inhibit directly the in vitro development of B. cinerea. Treatment of grapevine leaves by chitosan led to marked induction of lipoxygenase (LOX), phenylalanine ammonia-lyase (PAL) and chitinase activities, three markers of plant defense responses. Dose-response curves show that maximum defense reactions (PAL and chitinase activities) and strong reduction of B. cinerea infection were achieved with 75-150mgl−1 chitosan. However, greater concentrations of chitosan did not protect grapevine leaves with the same efficiency, but inhibited mycelial growth in vitro. Present results underlined the potency of chitosan in inducing some defense responses in grapevine leaves which in turn might improve resistance to grey mould. Springer, 2006 chitinase nationallicence elicitor nationallicence grey mould nationallicence lipoxygenase nationallicence Phe ammonia-lyase nationallicence Vitis vinifera nationallicence LOX : lipoxygenase nationallicence PAL : phenylalanine ammonia-lyase nationallicence PR : pathogenesis related nationallicence Trotel-Aziz Patricia Laboratoire d'Eco-Toxicologie, URVVC - EA 2069, Université de Reims Champagne-Ardenne, BP 1039, 51687, cedex 2, Reims, France aut Couderchet Michel Laboratoire d'Eco-Toxicologie, URVVC - EA 2069, Université de Reims Champagne-Ardenne, BP 1039, 51687, cedex 2, Reims, France aut Vernet Guy Laboratoire d'Eco-Toxicologie, URVVC - EA 2069, Université de Reims Champagne-Ardenne, BP 1039, 51687, cedex 2, Reims, France aut Aziz Aziz Laboratoire d'Eco-Toxicologie, URVVC - EA 2069, Université de Reims Champagne-Ardenne, BP 1039, 51687, cedex 2, Reims, France aut European Journal of Plant Pathology Kluwer Academic Publishers 114/4(2006-04-01), 405-413 0929-1873 114:4<405 2006 114 10658 https://doi.org/10.1007/s10658-006-0005-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-006-0005-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Trotel-Aziz Patricia Laboratoire d'Eco-Toxicologie, URVVC - EA 2069, Université de Reims Champagne-Ardenne, BP 1039, 51687, cedex 2, Reims, France aut NATIONALLICENCE 700 1- Couderchet Michel Laboratoire d'Eco-Toxicologie, URVVC - EA 2069, Université de Reims Champagne-Ardenne, BP 1039, 51687, cedex 2, Reims, France aut NATIONALLICENCE 700 1- Vernet Guy Laboratoire d'Eco-Toxicologie, URVVC - EA 2069, Université de Reims Champagne-Ardenne, BP 1039, 51687, cedex 2, Reims, France aut NATIONALLICENCE 700 1- Aziz Aziz Laboratoire d'Eco-Toxicologie, URVVC - EA 2069, Université de Reims Champagne-Ardenne, BP 1039, 51687, cedex 2, Reims, France aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 114/4(2006-04-01), 405-413 0929-1873 114:4<405 2006 114 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer