caa a22 4500 46792936X CHVBK 20180406152939.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s10658-006-0003-7 doi (NATIONALLICENCE)springer-10.1007/s10658-006-0003-7 Characterization of Erwinia amylovora Strains from Croatia [Elektronische Daten] [Edyta Halupecki, Carlo Bazzi, Susanne Jock, Klaus Geider, Damir Đermić, Bogdan Cvjetković] Erwinia amylovora is the causative agent of fire blight, a destructive disease of rosaceous plants. The European population can be divided into several subtypes according to differences in restriction fragment length polymorphism of the XbaI genomic DNA digest analysed with pulsed-field gel electrophoresis. This technique was also used to determine the genetic relatedness of six Croatian isolates to the E. amylovora types found in the countries surrounding Croatia. The isolates belong to the Pt2 pattern type that is characteristic of the East Mediterranean basin. All tested isolates gave essentially the same total cell protein pattern in SDS-polyacrylamide gel electrophoresis. The number of short-sequence DNA repeats in plasmid pEA29 of six isolates was determined by PCR assays and ranged from four to seven. The isolates examined showed high pathogenicity in immature pear fruits. Differences were also revealed in microbiological assays such as amylovoran synthesis, levan formation, siderophore production and colour on coliform medium. Springer, 2006 fire blight nationallicence pathogenicity nationallicence PFGE nationallicence SSR nationallicence Halupecki Edyta Department of Plant Pathology, Faculty of Agriculture, Svetošimunska 25, 10000, Zagreb, Croatia aut Bazzi Carlo Department of Agroenvironmental Sciences and Technologies, University of Bologna, Viale Fanin 42, 40127, Bologna, Italy aut Jock Susanne Max Planck Institute Ladenburg, Schwabenheimer Str. 101, D-69221, Dossenheim, Germany aut Geider Klaus Max Planck Institute Ladenburg, Schwabenheimer Str. 101, D-69221, Dossenheim, Germany aut Đermić Damir Department of Molecular Biology, Ruđer Bošković Institute, Bijenička 54, 10000, Zagreb, Croatia aut Cvjetković Bogdan Department of Plant Pathology, Faculty of Agriculture, Svetošimunska 25, 10000, Zagreb, Croatia aut European Journal of Plant Pathology Kluwer Academic Publishers 114/4(2006-04-01), 435-440 0929-1873 114:4<435 2006 114 10658 https://doi.org/10.1007/s10658-006-0003-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-006-0003-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Halupecki Edyta Department of Plant Pathology, Faculty of Agriculture, Svetošimunska 25, 10000, Zagreb, Croatia aut NATIONALLICENCE 700 1- Bazzi Carlo Department of Agroenvironmental Sciences and Technologies, University of Bologna, Viale Fanin 42, 40127, Bologna, Italy aut NATIONALLICENCE 700 1- Jock Susanne Max Planck Institute Ladenburg, Schwabenheimer Str. 101, D-69221, Dossenheim, Germany aut NATIONALLICENCE 700 1- Geider Klaus Max Planck Institute Ladenburg, Schwabenheimer Str. 101, D-69221, Dossenheim, Germany aut NATIONALLICENCE 700 1- Đermić Damir Department of Molecular Biology, Ruđer Bošković Institute, Bijenička 54, 10000, Zagreb, Croatia aut NATIONALLICENCE 700 1- Cvjetković Bogdan Department of Plant Pathology, Faculty of Agriculture, Svetošimunska 25, 10000, Zagreb, Croatia aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 114/4(2006-04-01), 435-440 0929-1873 114:4<435 2006 114 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer