caa a22 4500 467929378 CHVBK 20180406152939.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s10658-005-2232-6 doi (NATIONALLICENCE)springer-10.1007/s10658-005-2232-6 Biological Control of Sclerotinia Diseases of Rapeseed by Aerial Applications of the Mycoparasite Coniothyrium minitans [Elektronische Daten] [G. Li, H. Huang, H. Miao, R. Erickson, D. Jiang, Y. Xiao] Indoor and field experiments were conducted to evaluate the efficacy of applying the mycoparasite Coniothyrium minitans to the aerial parts of rapeseed plants at the flowering stage to control sclerotinia diseases caused by Sclerotinia sclerotiorum. Under controlled conditions, a petal inoculation technique was used to determine the effect of conidial suspensions of C. minitans on suppression of sclerotinia leaf blight. Results showed that C. minitans was effective in inhibiting infection initiated by ascospores of S. sclerotiorum on flower petals by restricting mycelial growth of the pathogen. Suppression of lesion development was related to the conidial concentration of C. minitans, with larger lesions at low concentration (5×103conidiaml−1), but smaller lesions at high concentration (5×104 conidiaml−1 or higher). When C. minitans-treated rapeseed leaves were inoculated with mycelia of S. sclerotiorum, C. minitans failed to prevent infection of leaves, but caused a significant reduction in number of sclerotia produced on the diseased leaves. No significant difference in efficacy was detected between the two isolates of C. minitans, LRC 2137 and Chy-1, on the two rapeseed cultivars, Westar (spring type) and Zhongyou 821 (winter type). Results of field trials showed a significant reduction of stem rot of rapeseed in four (1997, 1999, 2003 and 2004) out of five years by aerial application of C. minitans, compared with controls. No significant difference in suppressive efficacy was observed between the treatments of C. minitans (106 conidiaml−1), C. minitans (106 conidiaml−1) + benomyl (50μgml−1) and benomyl (100μgml−1) in 2003, and between the treatments of C. minitans (106 conidiaml−1), C. minitans (106 conidiaml−1) + vinclozolin (100μgml−1) and vinclozolin (500μgml−1) in 2004. Sclerotia of S. sclerotiorum collected from diseased plants in plots treated with C. minitans in 1999, 2000 and 2003, or with C. minitans + benomyl in 2003 were infected by C. minitans at frequencies ranging from 21.3 to 54.5%. This study concludes that aerial spraying of C. minitans is an effective method for controlling sclerotinia diseases of rapeseed. Springer, 2006 aerial application nationallicence biological control nationallicence Brassica napus nationallicence Coniothyrium minitans nationallicence foliar spray nationallicence phylloplane nationallicence Sclerotinia sclerotiorum nationallicence Li G. Department of Plant Protection, Huazhong Agricultural University, 430070, Wuhan, China aut Huang H. Research Centre, Agriculture and Agri-Food Canada, T1J 4B1, Lethbridge, Alberta, Canada aut Miao H. Department of Plant Protection, Huazhong Agricultural University, 430070, Wuhan, China aut Erickson R. Research Centre, Agriculture and Agri-Food Canada, T1J 4B1, Lethbridge, Alberta, Canada aut Jiang D. Department of Plant Protection, Huazhong Agricultural University, 430070, Wuhan, China aut Xiao Y. Department of Plant Protection, Huazhong Agricultural University, 430070, Wuhan, China aut European Journal of Plant Pathology Kluwer Academic Publishers 114/4(2006-04-01), 345-355 0929-1873 114:4<345 2006 114 10658 https://doi.org/10.1007/s10658-005-2232-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-005-2232-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li G. Department of Plant Protection, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Huang H. Research Centre, Agriculture and Agri-Food Canada, T1J 4B1, Lethbridge, Alberta, Canada aut NATIONALLICENCE 700 1- Miao H. Department of Plant Protection, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Erickson R. Research Centre, Agriculture and Agri-Food Canada, T1J 4B1, Lethbridge, Alberta, Canada aut NATIONALLICENCE 700 1- Jiang D. Department of Plant Protection, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Xiao Y. Department of Plant Protection, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 114/4(2006-04-01), 345-355 0929-1873 114:4<345 2006 114 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer