caa a22 4500 467929394 CHVBK 20180406152939.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s10658-006-0004-6 doi (NATIONALLICENCE)springer-10.1007/s10658-006-0004-6 Detection and Elimination of Viruses in Callus, Somatic Embryos and Regenerated Plantlets of Grapevine [Elektronische Daten] [Giorgio Gambino, Jeannette Bondaz, Ivana Gribaudo] The distribution of some grapevine viruses in flower explants, embryogenic and non-embryogenic calli, single somatic embryos and plants regenerated from embryogenic cultures was investigated by RT-PCR and ELISA. Immature anthers and ovaries of the cultivars Grignolino infected by GRSPaV, GLRaV-1 and GVA, Müller-Thurgau infected by GRSPaV and GLRaV-3 and Bosco infected by GRSPaV were cultivated on media inducing indirect somatic embryogenesis. Viruses were detected both in anthers and ovaries. Four months after culture initiation 65.6% of tested calli were infected by at least one virus; high percentages of virus infection were found in calli originating from ovaries. No virus was detected in calli tested 8months after culture initiation, as well as in single somatic embryos or in embryo-derived plantlets. Somatic embryogenesis confirmed its effectiveness in eliminating phloem-limited grapevine viruses. Regeneration of RT-PCR negative plantlets occurred even when at least a sector of the callus was still infected: the mechanism whereby somatic embryos are freed of some viruses could be related to the rapid proliferation of embryogenic cells within the callus or to the origin of the embryogenic callus from virus-free cells within the original explant. Springer, 2006 grapevine nationallicence somatic embryogenesis nationallicence virus eradication nationallicence Vitis vinifera nationallicence Gambino Giorgio Viticulture Unit, Plant Virology Institute CNR (IVV), Via L. da Vinci 44, I-10095, Grugliasco-TO, Italy aut Bondaz Jeannette Viticulture Unit, Plant Virology Institute CNR (IVV), Via L. da Vinci 44, I-10095, Grugliasco-TO, Italy aut Gribaudo Ivana Viticulture Unit, Plant Virology Institute CNR (IVV), Via L. da Vinci 44, I-10095, Grugliasco-TO, Italy aut European Journal of Plant Pathology Kluwer Academic Publishers 114/4(2006-04-01), 397-404 0929-1873 114:4<397 2006 114 10658 https://doi.org/10.1007/s10658-006-0004-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-006-0004-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gambino Giorgio Viticulture Unit, Plant Virology Institute CNR (IVV), Via L. da Vinci 44, I-10095, Grugliasco-TO, Italy aut NATIONALLICENCE 700 1- Bondaz Jeannette Viticulture Unit, Plant Virology Institute CNR (IVV), Via L. da Vinci 44, I-10095, Grugliasco-TO, Italy aut NATIONALLICENCE 700 1- Gribaudo Ivana Viticulture Unit, Plant Virology Institute CNR (IVV), Via L. da Vinci 44, I-10095, Grugliasco-TO, Italy aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 114/4(2006-04-01), 397-404 0929-1873 114:4<397 2006 114 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer