caa a22 4500 467929483 CHVBK 20180406152939.0 cr unu---uuuuu 170328e20060201xx s 000 0 eng 10.1007/s10658-005-2333-2 doi (NATIONALLICENCE)springer-10.1007/s10658-005-2333-2 Plant Host Range of Verticillium longisporum and Microsclerotia Density in Swedish Soils [Elektronische Daten] [Anna Johansson, Jan-Kees Goud, Christina Dixelius] Verticillium longisporum is a soil-borne fungal pathogen causing vascular wilt of Brassica crops. This study was conducted to enhance our knowledge on the host range of V. longisporum. Seven crop species (barley, oat, oilseed rape, pea, red clover, sugar beet and wheat) and five weed species (barren brome, black-grass, charlock, cleavers and scentless mayweed) all common in southern Sweden were evaluated for infection by response to V. longisporum. Oat, spring wheat, oilseed rape, scentless mayweed and charlock inoculated with V. longisporum in a greenhouse showed stunting to various degrees close to the fully ripe stage. Based on the extent of microsclerotia formation, explants were separated into four groups: for pea and wheat, <5% of the samples had formed microsclerotia; for scentless mayweed, 5-10%; for oat, 10-20%; and for charlock and oilseed rape >80%. The results suggest that plant species outside the Brassicaceae can act as reservoirs of V. longisporum inoculum. Soil inoculum densities in nine fields were monitored over a period of 12months, which ranged from 1 to 48cfu g−1 soil. Density of microsclerotia was lowest just after harvest, reaching its maximum six months later. No significant correlation between inoculum density in soil and disease incidence on oilseed rape plants was found. However, the data suggest that a threshold of 1cfu g−1 soil is needed to cause disease on oilseed rape. Species identification based on microsclerotia morphology and PCR analysis showed that V. longisporum dominated in soil of seven, and V. dahliae in two of the nine fields studied. Springer, 2006 Brassica nationallicence Verticillium dahliae nationallicence Verticillium wilt nationallicence weeds nationallicence Johansson Anna Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, P.O.Box 7080S-75007, Uppsala, Sweden aut Goud Jan-Kees Biological Farming Systems Group, Department of Plant Sciences, Wageningen University, Maryjkeweg 22, 6709, Wageningen, PG, The Netherlands aut Dixelius Christina Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, P.O.Box 7080S-75007, Uppsala, Sweden aut European Journal of Plant Pathology Kluwer Academic Publishers 114/2(2006-02-01), 139-149 0929-1873 114:2<139 2006 114 10658 https://doi.org/10.1007/s10658-005-2333-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-005-2333-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Johansson Anna Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, P.O.Box 7080S-75007, Uppsala, Sweden aut NATIONALLICENCE 700 1- Goud Jan-Kees Biological Farming Systems Group, Department of Plant Sciences, Wageningen University, Maryjkeweg 22, 6709, Wageningen, PG, The Netherlands aut NATIONALLICENCE 700 1- Dixelius Christina Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, P.O.Box 7080S-75007, Uppsala, Sweden aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 114/2(2006-02-01), 139-149 0929-1873 114:2<139 2006 114 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer