caa a22 4500 467929629 CHVBK 20180406152939.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s10658-006-9025-4 doi (NATIONALLICENCE)springer-10.1007/s10658-006-9025-4 Potential Inoculum Sources of Phaeomoniella chlamydospora in South African Grapevine Nurseries [Elektronische Daten] [E. Retief, A. McLeod, P. Fourie] Petri disease of grapevine is primarily caused by Phaeomoniella chlamydospora. This pathogen affects mostly young grapevines, but is also implicated in esca disease of older grapevines. Little is known about the disease cycle of this fungus. Infected propagation material was identified as a major means of dissemination of the pathogen. Recently, the pathogen was also detected from soil in South Africa and airborne conidia have been found in vineyards. The aim of this study was to use a molecular detection technique to test different samples collected from nurseries in South Africa at different nursery stages for the presence of P. chlamydospora. A one-tube nested-PCR technique was optimised for detecting P. chlamydospora in DNA extracted from soil, water, callusing medium and grapevine wood. The one-tube nested-PCR was sensitive enough to detect as little as 1fg of P. chlamydospora genomic DNA from water and 10fg from wood, callusing medium and soil. PCR analyses of the different nursery samples revealed the presence of several putative 360bp P. chlamydospora specific bands. Subsequent sequence analyses and/or restriction enzyme digestions of all 360bp PCR bands confirmed that all bands were P. chlamydospora specific, except for five bands obtained from callusing media and one from water. Phaeomoniella chlamydospora was positively detected in 25% of rootstock cane sections collected from mother blocks, 42% of rootstock cuttings and 16% of scion cuttings collected during grafting, 40% of water samples collected after pre-storage hydration, 67% of water samples collected during grafting, 8% of callusing medium samples and 17% of soil samples collected from mother blocks. These media can therefore be considered as possible inoculum sources of the pathogen during the nursery stages. Springer Science+Business Media, Inc., 2006 black goo nationallicence molecular detection nationallicence one-tube nested-PCR nationallicence petri disease nationallicence Retief E. Department of Plant Pathology, University of Stellenbosch, Private Bag X1, 7602, Stellenbosch, South Africa aut McLeod A. Department of Plant Pathology, University of Stellenbosch, Private Bag X1, 7602, Stellenbosch, South Africa aut Fourie P. Department of Plant Pathology, University of Stellenbosch, Private Bag X1, 7602, Stellenbosch, South Africa aut European Journal of Plant Pathology Kluwer Academic Publishers 115/3(2006-07-01), 331-339 0929-1873 115:3<331 2006 115 10658 https://doi.org/10.1007/s10658-006-9025-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-006-9025-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Retief E. Department of Plant Pathology, University of Stellenbosch, Private Bag X1, 7602, Stellenbosch, South Africa aut NATIONALLICENCE 700 1- McLeod A. Department of Plant Pathology, University of Stellenbosch, Private Bag X1, 7602, Stellenbosch, South Africa aut NATIONALLICENCE 700 1- Fourie P. Department of Plant Pathology, University of Stellenbosch, Private Bag X1, 7602, Stellenbosch, South Africa aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 115/3(2006-07-01), 331-339 0929-1873 115:3<331 2006 115 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer