caa a22 4500 467929661 CHVBK 20180406152939.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s10658-006-9018-3 doi (NATIONALLICENCE)springer-10.1007/s10658-006-9018-3 The Use of Bioluminescence for Monitoring in planta Growth Dynamics of a Pseudomonas syringae Plant Pathogen [Elektronische Daten] [Christopher Paynter, Vyvyan Salisbury, Dawn Arnold, Robert Jackson] The use of bioluminescence was evaluated as a tool to study Pseudomonas syringae population dynamics in susceptible and resistant plant environments. Plasmid pGLITE, containing the luxCDABE genes from Photorhabdus luminescens, was introduced into Pseudomonas syringae pv. phaseolicola race 7 strain 1449B, a Gram-negative pathogen of bean (Phaseolus vulgaris). Bacteria recovered from plant tissue over a five-day period were enumerated by counting numbers of colony forming units and by measurement of bioluminescence. Direct measurement of bioluminescence from leaf disc homogenates consistently reflected bacterial growth as determined by viable counting, but also detected subtle effects of the plant resistance response on bacterial viability. This bioluminescence procedure enables real time measurement of bacterial metabolism and population dynamics in planta, obviates the need to carry out labour intensive and time consuming traditional enumeration techniques and provides a sensitive assay for studying plant effects on bacterial cells. Springer, 2006 Avirulence nationallicence bacteria nationallicence bean nationallicence lux nationallicence pathogenicity nationallicence Paynter Christopher Faculty of Applied Sciences, University of the West of England, Coldharbour Lane, BS16 1QY, Frenchay, Bristol, UK aut Salisbury Vyvyan Faculty of Applied Sciences, University of the West of England, Coldharbour Lane, BS16 1QY, Frenchay, Bristol, UK aut Arnold Dawn Faculty of Applied Sciences, University of the West of England, Coldharbour Lane, BS16 1QY, Frenchay, Bristol, UK aut Jackson Robert Faculty of Applied Sciences, University of the West of England, Coldharbour Lane, BS16 1QY, Frenchay, Bristol, UK aut European Journal of Plant Pathology Kluwer Academic Publishers 115/3(2006-07-01), 363-366 0929-1873 115:3<363 2006 115 10658 https://doi.org/10.1007/s10658-006-9018-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-006-9018-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Paynter Christopher Faculty of Applied Sciences, University of the West of England, Coldharbour Lane, BS16 1QY, Frenchay, Bristol, UK aut NATIONALLICENCE 700 1- Salisbury Vyvyan Faculty of Applied Sciences, University of the West of England, Coldharbour Lane, BS16 1QY, Frenchay, Bristol, UK aut NATIONALLICENCE 700 1- Arnold Dawn Faculty of Applied Sciences, University of the West of England, Coldharbour Lane, BS16 1QY, Frenchay, Bristol, UK aut NATIONALLICENCE 700 1- Jackson Robert Faculty of Applied Sciences, University of the West of England, Coldharbour Lane, BS16 1QY, Frenchay, Bristol, UK aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 115/3(2006-07-01), 363-366 0929-1873 115:3<363 2006 115 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer