caa a22 4500 467929734 CHVBK 20180406152940.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s10658-006-9032-5 doi (NATIONALLICENCE)springer-10.1007/s10658-006-9032-5 Evaluation of the API 50CH and API ZYM systems for rapid characterization of Clavibacter michiganensis subsp. sepedonicus , causal agent of potato ring rot [Elektronische Daten] [José Palomo, María López, Pablo García-Benavides, Encarna Velázquez, Eustoquio Martínez-Molina] API 50CH and API ZYM systems were used to characterize fifty-three strains of Clavibacter michiganensis subsp. sepedonicus from different geographic locations and several reference strains of the same and different species, including other potato pathogens. Clavibacter michiganensis subsp. sepedonicus strains displayed a high level of homogeneity, both in carbohydrate utilization and in enzymatic activity. Using API 50CH and API ZYM it was possible to differentiate C. michiganensis subsp. sepedonicus strains from the remaining taxa analysed in this study, which included representative strains of the other subspecies of C. michiganensis as well as other bacterial pathogens affecting potatoes. Therefore, these systems could be used as an effective method to characterize C. michiganensis subsp. sepedonicus. Such a procedure would constitute an alternative system to the conventional nutritional and physiological identification tests currently included in the official methods employed in the European Union to detect and identify this bacterium. The results obtained with the API systems agreed with the current taxonomic classification of C. michiganensis, clearly separating sepedonicus from the other subspecies belonging to this species. Springer Science+Business Media, Inc., 2006 Clavibacter nationallicence characterization nationallicence miniaturized systems nationallicence potato pathogens nationallicence Palomo José Centro Regional de Diagnóstico, Junta de Castilla y León, Apdo. 61, 37080, Salamanca, Spain aut López María Instituto Valenciano de Investigaciones Agrarias, Ctra. Moncada-Náquera Km. 4,5, 46113, Moncada, Valencia, Spain aut García-Benavides Pablo Centro Regional de Diagnóstico, Junta de Castilla y León, Apdo. 61, 37080, Salamanca, Spain aut Velázquez Encarna Departamento de Microbiología y Genética. Lab. 209. Edificio Departamental, Universidad de Salamanca, Campus Unamuno, 37007, Salamanca, Spain aut Martínez-Molina Eustoquio Departamento de Microbiología y Genética. Lab. 209. Edificio Departamental, Universidad de Salamanca, Campus Unamuno, 37007, Salamanca, Spain aut European Journal of Plant Pathology Kluwer Academic Publishers 115/4(2006-08-01), 443-451 0929-1873 115:4<443 2006 115 10658 https://doi.org/10.1007/s10658-006-9032-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-006-9032-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Palomo José Centro Regional de Diagnóstico, Junta de Castilla y León, Apdo. 61, 37080, Salamanca, Spain aut NATIONALLICENCE 700 1- López María Instituto Valenciano de Investigaciones Agrarias, Ctra. Moncada-Náquera Km. 4,5, 46113, Moncada, Valencia, Spain aut NATIONALLICENCE 700 1- García-Benavides Pablo Centro Regional de Diagnóstico, Junta de Castilla y León, Apdo. 61, 37080, Salamanca, Spain aut NATIONALLICENCE 700 1- Velázquez Encarna Departamento de Microbiología y Genética. Lab. 209. Edificio Departamental, Universidad de Salamanca, Campus Unamuno, 37007, Salamanca, Spain aut NATIONALLICENCE 700 1- Martínez-Molina Eustoquio Departamento de Microbiología y Genética. Lab. 209. Edificio Departamental, Universidad de Salamanca, Campus Unamuno, 37007, Salamanca, Spain aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 115/4(2006-08-01), 443-451 0929-1873 115:4<443 2006 115 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer