caa a22 4500 467929785 CHVBK 20180406152940.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s10658-006-9031-6 doi (NATIONALLICENCE)springer-10.1007/s10658-006-9031-6 Biocontrol agents in combination with Moringa oleifera extract for integrated control of Sclerotium- caused cowpea damping-off and stem rot [Elektronische Daten] [A. Adandonon, T.A.S. Aveling, N. Labuschagne, M. Tamo] Damping-off and stem rot disease-causing Sclerotium rolfsii has been reported as a destructive soil-borne pathogen of numerous crops, especially in the tropics and subtropics. Trials were conducted to test the efficacy of biocontrol agents alone or combined with Moringa oleifera leaf extracts for the control of the disease. In the laboratory, PDA was amended with Moringa leaf extract, and mycelial growth of S. rolfsii was measured. In the greenhouse and field, Trichoderma Kd 63, Trichoderma IITA 508 and Bacillus subtilis were evaluated as seed treatments, soil drench or sprinkle, separately or combined with Moringa leaf extracts. Percentage disease incidence, severity and control were recorded. In the laboratory, the higher the extract concentration the less the mycelial growth and no mycelial growth occurred on extract at 15 or 20g leaves 10ml−1 water. In the greenhouse, the highest disease control was observed at a Moringa extract concentration of 15kg leaves 10l−1 water (w/v). Seed treatments using Trichoderma Kd 63, and soil sprinkle using Trichoderma IITA 508 had a significantly (P=0.05) higher effect on a disease incidence than Bacillus. Disease severity followed the same pattern. Moringa seed treatment combined with Trichoderma soil sprinkle resulted in significantly more than 94% and 70% disease control in the greenhouse and field, respectively, with significant yield increase in the field. This is the first report of Moringa leaf extract combined with Trichoderma as an integrated control for Sclerotium damping-off and stem rot of cowpea in the field. Springer Science+Business Media, Inc., 2006 Bacillus subtilis nationallicence integrated control nationallicence Moringa oleifera nationallicence Sclerotium rolfsii nationallicence Trichoderma harzianum nationallicence Vigna unguiculata nationallicence Adandonon A. National Institute of Agricultural Research (INRAB), Cotonou, Benin aut Aveling T.A.S. Department of Microbiology and␣Plant Pathology, University of Pretoria, Pretoria, South Africa aut Labuschagne N. Department of Microbiology and␣Plant Pathology, University of Pretoria, Pretoria, South Africa aut Tamo M. International Institute of Tropical Agriculture (IITA), Cotonou, Benin aut European Journal of Plant Pathology Kluwer Academic Publishers 115/4(2006-08-01), 409-418 0929-1873 115:4<409 2006 115 10658 https://doi.org/10.1007/s10658-006-9031-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-006-9031-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Adandonon A. National Institute of Agricultural Research (INRAB), Cotonou, Benin aut NATIONALLICENCE 700 1- Aveling T.A.S. Department of Microbiology and␣Plant Pathology, University of Pretoria, Pretoria, South Africa aut NATIONALLICENCE 700 1- Labuschagne N. Department of Microbiology and␣Plant Pathology, University of Pretoria, Pretoria, South Africa aut NATIONALLICENCE 700 1- Tamo M. International Institute of Tropical Agriculture (IITA), Cotonou, Benin aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 115/4(2006-08-01), 409-418 0929-1873 115:4<409 2006 115 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer