caa a22 4500 467929807 CHVBK 20180406152940.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s10658-006-9012-9 doi (NATIONALLICENCE)springer-10.1007/s10658-006-9012-9 Genetic Structure of the Population of Phytophthora infestans Attacking Solanum ochranthum in the Highlands of Ecuador [Elektronische Daten] [M. Chacón, N. Adler, F. Jarrin, W. Flier, C. Gessler, G. Forbes] Thirty-nine isolates of Phytophthora infestans were collected from the wild host Solanum ochranthum in the highland tropics of Ecuador and characterized with a set of phenotypic and molecular markers (mating type, metalaxyl sensitivity, the allozyme loci Gpi, and Pep, mitochondrial DNA haplotype, RFLP, and SSR), as well as for pathogenicity on various hosts. Three groups of isolates (A, B, and C) were identified based on their multilocus genotypes and variable abilities to cause disease on different hosts. Group A had a combination of alleles for the Gpi (86/100), Pep (96/100) and mtDNA (Ia) loci, as well as an RFLP fingerprint, that have not been reported for P. infestans in Ecuador, or elsewhere. Group B shares many marker characteristics with the US-1 lineage described in Ecuador on tomato, pear melon (S. muricatum), and S. caripense, but has SSR alleles not present in typical US-1 isolates. Group C for all markers tested is identical to the EC-1 lineage described on cultivated and wild potatoes in Ecuador. All isolates from S. ochranthum were able to re-infect their host of origin in the detached leaf assay; however, we did not draw clear conclusions as to the relative aggressiveness of the three groups on this host. Isolates of group A were the most specialized and were generally non-pathogenic or weakly pathogenic on all hosts other than S. ochranthum. Groups B and C infected tuber-bearing hosts, including the cultivated potato but were generally non-pathogenic on other non-tuber bearing hosts. Solanum ochranthum was infected by isolates coming from tuber-bearing Solanum hosts (i.e., the EC-1 lineage of P. infestans) and some US-1 isolates from non-tuber bearing hosts. Thus, in nature this species might be a potential reservoir of inoculum of different pathogen populations able to infect the cultivated hosts potato, tomato and pear melon (S.␣muricatum). Unlike potato and tomato in Ecuador, each of which is primarily attacked by a highly specialized pathogen population, S. ochranthum appears to harbour at least three pathogen groups of␣different genetic make-up. The unresolved issue of potential host specificity in isolates found on S.␣ochranthum could complicate efforts to use this species in tomato improvement. Springer, 2006 Solanum ochranthum nationallicence Ecuador nationallicence wild potato species nationallicence SSR nationallicence multilocus genotype nationallicence pathogenicity nationallicence Chacón M. International Potato Center (CIP), 17-21-1977, Quito, Ecuador aut Adler N. International Potato Center (CIP), 17-21-1977, Quito, Ecuador aut Jarrin F. International Potato Center (CIP), 17-21-1977, Quito, Ecuador aut Flier W. Fine Agrochemicals Ltd., Cuneralaan 31 3911AA, Rhenen, The Netherlands aut Gessler C. Department Plant Pathology, Swiss Federal Institute of Technology Zurich, CH-8092, Zürich, ETH, Switzerland aut Forbes G. International Potato Center (CIP), Apartado 1558, 12, Lima, Peru aut European Journal of Plant Pathology Kluwer Academic Publishers 115/2(2006-06-01), 235-245 0929-1873 115:2<235 2006 115 10658 https://doi.org/10.1007/s10658-006-9012-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-006-9012-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chacón M. International Potato Center (CIP), 17-21-1977, Quito, Ecuador aut NATIONALLICENCE 700 1- Adler N. International Potato Center (CIP), 17-21-1977, Quito, Ecuador aut NATIONALLICENCE 700 1- Jarrin F. International Potato Center (CIP), 17-21-1977, Quito, Ecuador aut NATIONALLICENCE 700 1- Flier W. Fine Agrochemicals Ltd., Cuneralaan 31 3911AA, Rhenen, The Netherlands aut NATIONALLICENCE 700 1- Gessler C. Department Plant Pathology, Swiss Federal Institute of Technology Zurich, CH-8092, Zürich, ETH, Switzerland aut NATIONALLICENCE 700 1- Forbes G. International Potato Center (CIP), Apartado 1558, 12, Lima, Peru aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 115/2(2006-06-01), 235-245 0929-1873 115:2<235 2006 115 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer