caa a22 4500 467929858 CHVBK 20180406152940.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s10658-006-9014-7 doi (NATIONALLICENCE)springer-10.1007/s10658-006-9014-7 Occurrence and distribution of resistance to QoI fungicides in populationsof Podosphaera fusca in south central Spain [Elektronische Daten] [D. Fernández-Ortuño, A. Pérez-García, F. López-Ruiz, D. Romero, A. de Vicente, J. Torés] Cucurbit powdery mildew caused by Podosphaera fusca limits crop production in Spain. Since its management is strongly dependent on chemicals, the rational design of control programmes requires a good understanding of the fungicide resistance phenomenon in field populations. Fifty single-spore isolates of P. fusca were tested for sensitivity to three quinone-outside inhibiting (QoI) fungicides: azoxystrobin, kresoxim-methyl and trifloxystrobin. Minimum inhibitory concentration (MIC) values for QoI-sensitive isolates were found to range from 0.25 to 10μgml−1 for azoxystrobin to 5-25μgml−1 for kresoxim-methyl, using a leaf disc-based bioassay. High levels of cross-resistance to QoI fungicides were found. Eleven isolates showed resistance to the three QoI fungicides tested with MIC and EC50 values >500μgml−1 resulting in RF values as high as >715 and >1000 for trifloxystrobin and azoxystrobin, respectively. A survey of P. fusca QoI resistance was carried out in different provinces located in the south central area of Spain during the cucurbit growing seasons in 2002, 2003 and 2004. Examination of a collection of 250 isolates for QoI resistance revealed that 32% were resistant to the three fungicides tested; the provinces of Ciudad Real, Córdoba and Murcia being the locations with the highest frequencies of resistance (44-74%). By contrast, no resistance was found in Badajoz, and relatively low frequencies were observed in Almería and Valencia (10-13%). Nearly 50% of resistant isolates were collected from melon plants. Based on these data, recommendations about the use of QoI fungicides for cucurbit powdery mildew management in the sampled areas are made. Springer Science+Business Media, Inc., 2006 chemical control nationallicence Cucurbitaceae nationallicence fungicide resistance nationallicence powdery mildew fungi nationallicence Qo-inhibitors nationallicence strobilurins nationallicence Fernández-Ortuño D. Estación Experimental "La Mayora” (CSIC), Algarrobo-Costa, E-29750, Málaga, Spain aut Pérez-García A. Grupo de Microbiología y Patología Vegetal-Unidad Asociada a CSIC, Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, E-29071, Málaga, Spain aut López-Ruiz F. Estación Experimental "La Mayora” (CSIC), Algarrobo-Costa, E-29750, Málaga, Spain aut Romero D. Grupo de Microbiología y Patología Vegetal-Unidad Asociada a CSIC, Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, E-29071, Málaga, Spain aut de Vicente A. Grupo de Microbiología y Patología Vegetal-Unidad Asociada a CSIC, Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, E-29071, Málaga, Spain aut Torés J. Estación Experimental "La Mayora” (CSIC), Algarrobo-Costa, E-29750, Málaga, Spain aut European Journal of Plant Pathology Kluwer Academic Publishers 115/2(2006-06-01), 215-222 0929-1873 115:2<215 2006 115 10658 https://doi.org/10.1007/s10658-006-9014-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-006-9014-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fernández-Ortuño D. Estación Experimental "La Mayora” (CSIC), Algarrobo-Costa, E-29750, Málaga, Spain aut NATIONALLICENCE 700 1- Pérez-García A. Grupo de Microbiología y Patología Vegetal-Unidad Asociada a CSIC, Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, E-29071, Málaga, Spain aut NATIONALLICENCE 700 1- López-Ruiz F. Estación Experimental "La Mayora” (CSIC), Algarrobo-Costa, E-29750, Málaga, Spain aut NATIONALLICENCE 700 1- Romero D. Grupo de Microbiología y Patología Vegetal-Unidad Asociada a CSIC, Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, E-29071, Málaga, Spain aut NATIONALLICENCE 700 1- de Vicente A. Grupo de Microbiología y Patología Vegetal-Unidad Asociada a CSIC, Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, E-29071, Málaga, Spain aut NATIONALLICENCE 700 1- Torés J. Estación Experimental "La Mayora” (CSIC), Algarrobo-Costa, E-29750, Málaga, Spain aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 115/2(2006-06-01), 215-222 0929-1873 115:2<215 2006 115 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer