caa a22 4500 467929890 CHVBK 20180406152940.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s10658-006-0010-8 doi (NATIONALLICENCE)springer-10.1007/s10658-006-0010-8 A Severe Hellenic CMV Tomato Isolate: Symptom Variability in Tobacco, Characterization and Discrimination of Variants [Elektronische Daten] [A. Sclavounos, A. Voloudakis, Ch. Arabatzis, P. Kyriakopoulou] A severe strain of Cucumber mosaic virus (CMV) originating from an infected tomato plant (Gastouni-Olympia, Greece) was isolated in tobacco (Nicotiana tabacum cv. Xanthi nc), after three serial local lesion passages in Chenopodium quinoa and designated CMV-G. CMV-G induces yellow mosaic (YM) symptoms in tobacco. When CMV-G was passed mechanically through C. quinoa, phenotypic variants inducing YM or green mild mosaic (MM) in tobacco were isolated. Aphid transmission, from different hosts, appears to be an effective approach for separating MM variants of CMV-G from YM variants. In particular, aphid transmission from zucchini proved to be very efficient in selecting for MM variants. In contrast, aphids transmitted only YM variants from tomato plants. Molecular characterization of CMV-G and its progeny resulted in their classification in the CMV subgroup IB, free of satellite RNA, being the first discovery of the subgroup IB in Greece. In the Solanaceae family (tobacco, tomato, pepper) YM variants induced more severe symptoms than the MM variants. YM and MM phenotype was stable in tobacco for all seven passages tried using the obtained YM and MM variants. Cross-protection experiments showed that an isolated MM variant was able to protect tobacco plants against a challenge infection by a YM variant. Springer, 2006 aphid transmission nationallicence cross protection nationallicence CMV nationallicence host range nationallicence symptom variability nationallicence CMV-variants nationallicence Sclavounos A. Department of Plant Pathology, Faculty of Crop Production, Agricultural University of Athens, Iera Odos 75, 11855, Athens, Hellas aut Voloudakis A. Laboratory of Plant Physiology, Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855, Athens, Hellas aut Arabatzis Ch Department of Plant Pathology, Faculty of Crop Production, Agricultural University of Athens, Iera Odos 75, 11855, Athens, Hellas aut Kyriakopoulou P. Department of Plant Pathology, Faculty of Crop Production, Agricultural University of Athens, Iera Odos 75, 11855, Athens, Hellas aut European Journal of Plant Pathology Kluwer Academic Publishers 115/2(2006-06-01), 163-172 0929-1873 115:2<163 2006 115 10658 https://doi.org/10.1007/s10658-006-0010-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-006-0010-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sclavounos A. Department of Plant Pathology, Faculty of Crop Production, Agricultural University of Athens, Iera Odos 75, 11855, Athens, Hellas aut NATIONALLICENCE 700 1- Voloudakis A. Laboratory of Plant Physiology, Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855, Athens, Hellas aut NATIONALLICENCE 700 1- Arabatzis Ch Department of Plant Pathology, Faculty of Crop Production, Agricultural University of Athens, Iera Odos 75, 11855, Athens, Hellas aut NATIONALLICENCE 700 1- Kyriakopoulou P. Department of Plant Pathology, Faculty of Crop Production, Agricultural University of Athens, Iera Odos 75, 11855, Athens, Hellas aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 115/2(2006-06-01), 163-172 0929-1873 115:2<163 2006 115 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer