caa a22 4500 467929947 CHVBK 20180406152940.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s10658-006-9002-y doi (NATIONALLICENCE)springer-10.1007/s10658-006-9002-y New Media for Increasing Sporulation and Germination of Phaeoisariopsis Griseola Conidia [Elektronische Daten] [Sebastian Stenglein, Monica Aulicino, Ana Arambarri, Pedro Balatti] The slow growth rate of the fungus Phaeoisariopsis griseola and the availability of a homogeneous highly concentrated inoculum is an important constraint for pathogenicity or virulence studies, where plant inoculations are needed. Therefore, the purpose of this paper was to evaluate the effect of supplementing culture media with Amaranthus cruentus seed meal on fungal growth and sporulation of isolates of P. griseola belonging to the Mesoamerican and Andean groups. The amendment of PDA or V8 media with A. cruentus seed meal resulted in a considerable increase in the number of conidia and also in their capacity to germinate; this depended mostly on the stage of maturity of conidia. Mesoamerican and Andean isolates produced a different number of conidia when cultured in vitro. Furthermore, while in Mesoamerican isolates a second degree polynomial represented the relationship between number of conidia and amount of A. cruentus supplementation, in Andean isolates the relationship was linear. It seems that either one or several of the nutritional factors provided by A. cruentus contributed to the increased production of conidia and their development, resulting in faster development of the disease and an earlier appearance of symptoms. Therefore, for cultural studies, especially for inoculum production and for pathogenicity evaluations, supplementation of the media with A. cruentus seed meal proved to be a good alternative. Springer, 2006 Amaranthus cruentus nationallicence Angular leaf spot nationallicence Supplemented media nationallicence Stenglein Sebastian Cátedra de Morfología Vegetal, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, CC 327, 1900, La Plata, Argentina aut Aulicino Monica Instituto Fitotécnico de Santa Catalina, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, Buenos Aires, Argentina aut Arambarri Ana Cátedra de Morfología Vegetal, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, CC 327, 1900, La Plata, Argentina aut Balatti Pedro Instituto de Fisiología Vegetal, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, CC 327, 1900, La Plata, Argentina aut European Journal of Plant Pathology Kluwer Academic Publishers 115/2(2006-06-01), 173-180 0929-1873 115:2<173 2006 115 10658 https://doi.org/10.1007/s10658-006-9002-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-006-9002-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Stenglein Sebastian Cátedra de Morfología Vegetal, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, CC 327, 1900, La Plata, Argentina aut NATIONALLICENCE 700 1- Aulicino Monica Instituto Fitotécnico de Santa Catalina, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Arambarri Ana Cátedra de Morfología Vegetal, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, CC 327, 1900, La Plata, Argentina aut NATIONALLICENCE 700 1- Balatti Pedro Instituto de Fisiología Vegetal, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, CC 327, 1900, La Plata, Argentina aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 115/2(2006-06-01), 173-180 0929-1873 115:2<173 2006 115 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer