caa a22 4500 467933103 CHVBK 20180406152949.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s10545-006-0253-8 doi (NATIONALLICENCE)springer-10.1007/s10545-006-0253-8 Hepatocyte transplantation: Studies in preclinical models [Elektronische Daten] [Anne Weber, Dominique Mahieu-Caputo, Michelle Hadchouel, Dominique Franco] Summary: Transplantation of allogeneic or genetically modified autologous hepatocytes may be an alternative to whole-liver transplantation for the treatment of hereditary metabolic liver diseases. Human hepatocytes have already been transplanted in patients, demonstrating the safety and feasibility of both approaches. Although a few cases of allogeneic transplantation have resulted in long-term engraftment and function, only a partial and transient correction of the disease was achieved. This may partly result from a lack of proliferation of transplanted cells. In rodents, transplanted hepatocytes do not proliferate in adult quiescent livers and repopulate recipient livers only when they display a proliferative advantage over resident hepatocytes. Most of these models are not transposable to humans, however. Our aim is to develop preclinical approaches to hepatocyte transplantation in nonhuman primates. We have defined a strategy that increases the engraftment efficiency of transplanted hepatocytes by inducing their proliferation together with that of resident hepatocytes. We have also immortalized simian fetal hepatic progenitor cells and shown that these cells do not proliferate in situ after transplantation into the livers of immunodeficient mice. By contrast early human hepatoblasts repopulate mouse livers more efficiently. However, if we consider the number of cells to be transplanted (one to several billion), the means of expanding and differentiating stem or progenitor cells other than hepatocytes will have to be determined prior to envisaging treating patients. SSIEM and Springer, 2006 Weber Anne Hôpital Bicêtre, Inserm U 804 and University Paris XI, le Kremlin-Bicêtre, France aut Mahieu-Caputo Dominique Hôpital Bicêtre, Inserm U 804 and University Paris XI, le Kremlin-Bicêtre, France aut Hadchouel Michelle Hôpital Bicêtre, Inserm U 804 and University Paris XI, le Kremlin-Bicêtre, France aut Franco Dominique Hôpital Bicêtre, Inserm U 804 and University Paris XI, le Kremlin-Bicêtre, France aut Journal of Inherited Metabolic Disease Kluwer Academic Publishers 29/2-3(2006-04-01), 436-441 0141-8955 29:2-3<436 2006 29 10545 https://doi.org/10.1007/s10545-006-0253-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10545-006-0253-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Weber Anne Hôpital Bicêtre, Inserm U 804 and University Paris XI, le Kremlin-Bicêtre, France aut NATIONALLICENCE 700 1- Mahieu-Caputo Dominique Hôpital Bicêtre, Inserm U 804 and University Paris XI, le Kremlin-Bicêtre, France aut NATIONALLICENCE 700 1- Hadchouel Michelle Hôpital Bicêtre, Inserm U 804 and University Paris XI, le Kremlin-Bicêtre, France aut NATIONALLICENCE 700 1- Franco Dominique Hôpital Bicêtre, Inserm U 804 and University Paris XI, le Kremlin-Bicêtre, France aut NATIONALLICENCE 773 0- Journal of Inherited Metabolic Disease Kluwer Academic Publishers 29/2-3(2006-04-01), 436-441 0141-8955 29:2-3<436 2006 29 10545 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer