caa a22 4500 467934584 CHVBK 20180406152953.0 cr unu---uuuuu 170328e20060201xx s 000 0 eng 10.1007/s00408-005-2557-0 doi (NATIONALLICENCE)springer-10.1007/s00408-005-2557-0 Matrix Metalloproteinase-1 Activation via Plasmin Generated on Alveolar Epithelial Cell Surfaces [Elektronische Daten] [Takashi Ishida, Hiroki Tsukada, Takashi Hasegawa, Hirohisa Yoshizawa, Fumitake Gejyo] Plasmin is a potent protease related to tissue repair/remodeling not by fibrinolysis alone but also by activation of cytokines such as transforming growth factor and hepatocyte growth factor and by activation of matrix metalloproteases. We examined whether matrix matalloproteinase-1 was activated via plasminogen activation on surfaces of cultured alveolar epithelial cells (A-549). Cells were cultured overnight with plasminogen, pro-matrix metalloproteinase-1, and type I collagen as a substrate. Sodium dodecil sulfate-polyacrylamide gel electrophoresis was used to detect type I collagen degradation in culture supernatant. Collagen degradation corresponded to cell surface plasmin generation. No such finding was seen in the absence of cells or plasminogen. Alveolar epithelial plasminogen activation is important in matrix metalloproteinase-1 activation and thus presumably in tissue remodeling in pulmonary fibrosing pulmonary diseases such as idiopathic pulmonary fibrosis. Springer Science+Business Media, Inc., 2006 Plasminogen nationallicence Plasmin nationallicence Alveolar epithelial cells nationallicence Matrix metalloproteinase nationallicence Tissue remodeling nationallicence Ishida Takashi Division of Respiratory Medicine, Niigata University Graduate School of Medical and Dental Sciences, 1-757 Asahimachi-dori, 951-8510, Niigata, Japan aut Tsukada Hiroki Division of Respiratory Medicine, Niigata University Graduate School of Medical and Dental Sciences, 1-757 Asahimachi-dori, 951-8510, Niigata, Japan aut Hasegawa Takashi Division of Respiratory Medicine, Niigata University Graduate School of Medical and Dental Sciences, 1-757 Asahimachi-dori, 951-8510, Niigata, Japan aut Yoshizawa Hirohisa Division of Respiratory Medicine, Niigata University Graduate School of Medical and Dental Sciences, 1-757 Asahimachi-dori, 951-8510, Niigata, Japan aut Gejyo Fumitake Division of Respiratory Medicine, Niigata University Graduate School of Medical and Dental Sciences, 1-757 Asahimachi-dori, 951-8510, Niigata, Japan aut Lung Springer-Verlag; www.springer-ny.com 184/1(2006-02-01), 15-19 0341-2040 184:1<15 2006 184 408 https://doi.org/10.1007/s00408-005-2557-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00408-005-2557-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ishida Takashi Division of Respiratory Medicine, Niigata University Graduate School of Medical and Dental Sciences, 1-757 Asahimachi-dori, 951-8510, Niigata, Japan aut NATIONALLICENCE 700 1- Tsukada Hiroki Division of Respiratory Medicine, Niigata University Graduate School of Medical and Dental Sciences, 1-757 Asahimachi-dori, 951-8510, Niigata, Japan aut NATIONALLICENCE 700 1- Hasegawa Takashi Division of Respiratory Medicine, Niigata University Graduate School of Medical and Dental Sciences, 1-757 Asahimachi-dori, 951-8510, Niigata, Japan aut NATIONALLICENCE 700 1- Yoshizawa Hirohisa Division of Respiratory Medicine, Niigata University Graduate School of Medical and Dental Sciences, 1-757 Asahimachi-dori, 951-8510, Niigata, Japan aut NATIONALLICENCE 700 1- Gejyo Fumitake Division of Respiratory Medicine, Niigata University Graduate School of Medical and Dental Sciences, 1-757 Asahimachi-dori, 951-8510, Niigata, Japan aut NATIONALLICENCE 773 0- Lung Springer-Verlag; www.springer-ny.com 184/1(2006-02-01), 15-19 0341-2040 184:1<15 2006 184 408 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer