caa a22 4500 467934746 CHVBK 20180406152953.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s00408-006-0009-0 doi (NATIONALLICENCE)springer-10.1007/s00408-006-0009-0 Comparative Genomic Hybridization Array Analysis and Real-Time PCR Reveals Genomic Copy Number Alteration for Lung Adenocarcinomas [Elektronische Daten] [Jin Choi, Long Zheng, Eunyoung Ha, Yun Lim, Yeul Kim, Young-Pil Wang, Young Lim] Genomic alterations in lung cancer tissues have been observed in various studies. To analyze the aberrations in the genome of lung cancer patients, we used array comparative genomic hybridization (array CGH) in 15 lung adenocarcinoma (AdC) tissues. Copy number gains and losses in chromosomal regions were detected and corresponding genes were confirmed by real-time polymerase chain reaction (PCR). As for the results, several frequently altered loci, including gain of 16p (46% of samples), were found, and the most common losses were found in 14q32.33 (26% of samples). High-level DNA amplifications (> 0.8 log2 ratio) were detected at 1p, 5p, 7p, 9p, 11p, 11q, 12q, 14q, 16p, 17q, 19q, 20p, 21q, and 22q. A subset of genes, gained or lost, was checked for over- or underrepresentation by means of real-time PCR. The degree of fold change was highest in ECGF1 (22q13.33), HOXA9 (7p15.2), MAFG (17q25.3), TSC2 (16p13.3), and ICAM1 (19p13.2) genes and the 16p chromosome terminal region (16p13.3pter). Taken together, these results show that array CGH could be used as a powerful tool for identification of genomic alteration for lung cancer, and the above-mentioned genes may represent potential candidate genes in the study of lung cancer pathogenesis and diagnosis. Springer Science+Business Media, Inc., 2006 Lung cancer nationallicence Adenocarcinoma nationallicence Array CGH nationallicence Real-time PCR nationallicence Choi Jin Catholic Neuroscience Center, The Catholic University, Seoul, Korea aut Zheng Long Department of Pharmacology College of Medicine, Kyung Hee University, Seoul, Korea aut Ha Eunyoung Department of Biochemistry College of Medicine, Kyung Hee University, Seoul, Korea aut Lim Yun Department of Internal Medicine, Dongguk University International Hospital, Dongguk University College of Medicine, Goyang, Korea aut Kim Yeul Department of Internal Medicine, Korea University Medical Center, Anam Hospital, Seoul, Korea aut Wang Young-Pil Department of Thoracic Surgery, The Catholic University, Seoul, Korea aut Lim Young Department of Occupational and Environmental Medicine, St. Mary's Hospital, The Catholic University, 150-713, Seoul, Korea aut Lung Springer-Verlag; www.springer-ny.com 184/6(2006-12-01), 355-362 0341-2040 184:6<355 2006 184 408 https://doi.org/10.1007/s00408-006-0009-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00408-006-0009-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Choi Jin Catholic Neuroscience Center, The Catholic University, Seoul, Korea aut NATIONALLICENCE 700 1- Zheng Long Department of Pharmacology College of Medicine, Kyung Hee University, Seoul, Korea aut NATIONALLICENCE 700 1- Ha Eunyoung Department of Biochemistry College of Medicine, Kyung Hee University, Seoul, Korea aut NATIONALLICENCE 700 1- Lim Yun Department of Internal Medicine, Dongguk University International Hospital, Dongguk University College of Medicine, Goyang, Korea aut NATIONALLICENCE 700 1- Kim Yeul Department of Internal Medicine, Korea University Medical Center, Anam Hospital, Seoul, Korea aut NATIONALLICENCE 700 1- Wang Young-Pil Department of Thoracic Surgery, The Catholic University, Seoul, Korea aut NATIONALLICENCE 700 1- Lim Young Department of Occupational and Environmental Medicine, St. Mary's Hospital, The Catholic University, 150-713, Seoul, Korea aut NATIONALLICENCE 773 0- Lung Springer-Verlag; www.springer-ny.com 184/6(2006-12-01), 355-362 0341-2040 184:6<355 2006 184 408 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer