caa a22 4500 467934886 CHVBK 20180406152954.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s00408-005-2585-9 doi (NATIONALLICENCE)springer-10.1007/s00408-005-2585-9 Structural Features of Epithelial Remodeling in Usual Interstitial Pneumonia Histologic Pattern [Elektronische Daten] [Aline Baptista, Edwin Parra, João Filho, Ronaldo Kairalla, Carlos de Carvalho, Vera Capelozzi] Epithelial remodeling probably contributes to parenchymal deterioration in usual interstitial pneumonia/idiopathic pulmonary fibrosis (UIP/IPF), but understanding its mechanisms is still a challenge. The aim of our study was to examine apoptosis and the epithelial changes in the histologic pattern of UIP. After immunohistochemical staining we quantified the content of type I cells, type II cells, surfactant-A protein, bcl-2, and Fas-ligand (Fas-L) in control and alveolar collapse, fibroblastic foci, and honeycomb in UIP areas of 23 open lung biopsies. A significant association was found between epithelial changes and parenchymal deterioration (p < 0.05). Type I epithelial cell density was similar between control (1.7 ± 0.7%) and UIP alveolar collapse areas (1.8 ± 0.6%) but decreased progressively in fibroblastic foci zones (0.8 ± 0.4%) and honeycomb changes (0.4 ± 0.3%). Type II cell density increased from control (25.6 ± 8.3%) to areas of alveolar collapse (34.5 ± 11.4%), then decreased toward fibroblastic foci (15.4 ± 6.0%) and honeycomb change areas (23.1 ± 8.6%). The surfactant-A protein increased from control (6.7 ± 3.2%) to areas of alveolar collapse (31.1 ± 9.5%) and decreased toward fibroblastic foci (14.5 ± 4.9%) and honeycomb change areas (21.1 ± 8.9%). Fas-L positive epithelial cell density presented a progressive decline from control (48.5 ± 9.5%), alveolar collapse (37.9 ± 12.4%), fibroblastic foci (27.4 ± 6.8%), and honeycomb change areas (21.9 ± 6.5%). A similar decline in density was found for bcl-2 positive epithelial cells from control (20.4 ± 2.7%), alveolar collapse (18.9 ± 5.1%), and fibroblastic foci areas (13.8 ±2.9%), then increased honeycomb change areas (16.3 ± 2.8%). We conclude that loss of the nuclear (bcl-2) and membrane (Fas-L) regulation of normal cell population density and suppression of cell death by apoptosis in UIP may be a determinant of the abnormal epithelial/parenchymal remodeling in UIP. Springer Science+Business Media, Inc., 2006 Usual interstitial pneumonia nationallicence Idiopathic pulmonary fibrosis nationallicence Surfactant-A Cytokerattn bcl -2 nationallicence Immuohistochemistry nationallicence Quantitation nationallicence Pathogenesis nationallicence Baptista Aline Department of Pathology, University of São Paulo Medical School, São Paulo, Brazil aut Parra Edwin Department of Pathology, University of São Paulo Medical School, São Paulo, Brazil aut Filho João Pulmonary Division, University of São Paulo Medical School, São Paulo, Brazil aut Kairalla Ronaldo Pulmonary Division, University of São Paulo Medical School, São Paulo, Brazil aut de Carvalho Carlos Pulmonary Division, University of São Paulo Medical School, São Paulo, Brazil aut Capelozzi Vera Department of Pathology, University of São Paulo Medical School, São Paulo, Brazil aut Lung Springer-Verlag; www.springer-ny.com 184/4(2006-08-01), 239-244 0341-2040 184:4<239 2006 184 408 https://doi.org/10.1007/s00408-005-2585-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00408-005-2585-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Baptista Aline Department of Pathology, University of São Paulo Medical School, São Paulo, Brazil aut NATIONALLICENCE 700 1- Parra Edwin Department of Pathology, University of São Paulo Medical School, São Paulo, Brazil aut NATIONALLICENCE 700 1- Filho João Pulmonary Division, University of São Paulo Medical School, São Paulo, Brazil aut NATIONALLICENCE 700 1- Kairalla Ronaldo Pulmonary Division, University of São Paulo Medical School, São Paulo, Brazil aut NATIONALLICENCE 700 1- de Carvalho Carlos Pulmonary Division, University of São Paulo Medical School, São Paulo, Brazil aut NATIONALLICENCE 700 1- Capelozzi Vera Department of Pathology, University of São Paulo Medical School, São Paulo, Brazil aut NATIONALLICENCE 773 0- Lung Springer-Verlag; www.springer-ny.com 184/4(2006-08-01), 239-244 0341-2040 184:4<239 2006 184 408 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer