caa a22 4500 467934894 CHVBK 20180406152954.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s00408-005-2588-6 doi (NATIONALLICENCE)springer-10.1007/s00408-005-2588-6 Immune Cell Infiltration and Broncovascular Remodeling After Nitric Acid Nasal Instillation in a Mouse Bronchiolitis Obliterans Model [Elektronische Daten] [A. L. Garippo, E. R. Parra, W. R. Teodoro, A. P. Veloza, N. H. Yoshinari, V. L. Capelozzi] Immune cell airway infiltration and the bronchovascular remodeling process have shown to be promising in the understanding of bronchiolitis obliterans (BO) pathogenesis. In this study we sought to validate the importance of immune cells, whether diffusely distributed or forming lymphoid follicles, collagen density, and vascular factors. Eight weeks after a single nitric acid (NA) nasal instillation, lung changes were characterized by lumen distortion, epithelial layer folding, reduction or total obliteration of terminal bronchiole (TB) lumen, and wall thickness increase. The morphologic changes in the TB and TA (terminal artery) lumen coincide with the measurement difference in the three groups. The TB diameter and lumen were significantly decreased in BO when compared with non-BO lungs (0.76 ± 0.05μm vs. 0.81 ± 0.05μm and 12286.13 ± 378.83μm vs. 18182.27 ± 5593.98μm, p = 0.05 and p = 0.01, respectively). Equally significant was the increase in TB thickness in BO when compared with the non-BO group (201.72 ± 35.75μm vs. 149.75 ± 40.61μm, p = 0.007). The morphologic changes in immune cells seen in TB, TA, and bronchus-associated lymphoid tissue (BALT) also coincide with the quantification differences observed in the three groups. We concluded that immune cell infiltration and collagen/vascular remodeling are related to the spectrum of histologic changes in a BO nasal-induced model in mice and may be an appropriate target for prospective studies of human bronchiolitis. Springer Science+Business Media, Inc., 2006 Bronchiolitis obliterans nationallicence Nitric acid nationallicence Immune cells nationallicence Collagen density and morphometry nationallicence Garippo A. L. Department of Pathology, School of Medicine, University of São Paulo, São Paulo, Brazil aut Parra E. R. Department of Pathology, School of Medicine, University of São Paulo, São Paulo, Brazil aut Teodoro W. R. Department of Rheumatology, School of Medicine, University of São Paulo, São Paulo, Brazil aut Veloza A. P. Department of Rheumatology, School of Medicine, University of São Paulo, São Paulo, Brazil aut Yoshinari N. H. Department of Rheumatology, School of Medicine, University of São Paulo, São Paulo, Brazil aut Capelozzi V. L. Department of Pathology, School of Medicine, University of São Paulo, São Paulo, Brazil aut Lung Springer-Verlag; www.springer-ny.com 184/4(2006-08-01), 229-238 0341-2040 184:4<229 2006 184 408 https://doi.org/10.1007/s00408-005-2588-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00408-005-2588-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Garippo A. L. Department of Pathology, School of Medicine, University of São Paulo, São Paulo, Brazil aut NATIONALLICENCE 700 1- Parra E. R. Department of Pathology, School of Medicine, University of São Paulo, São Paulo, Brazil aut NATIONALLICENCE 700 1- Teodoro W. R. Department of Rheumatology, School of Medicine, University of São Paulo, São Paulo, Brazil aut NATIONALLICENCE 700 1- Veloza A. P. Department of Rheumatology, School of Medicine, University of São Paulo, São Paulo, Brazil aut NATIONALLICENCE 700 1- Yoshinari N. H. Department of Rheumatology, School of Medicine, University of São Paulo, São Paulo, Brazil aut NATIONALLICENCE 700 1- Capelozzi V. L. Department of Pathology, School of Medicine, University of São Paulo, São Paulo, Brazil aut NATIONALLICENCE 773 0- Lung Springer-Verlag; www.springer-ny.com 184/4(2006-08-01), 229-238 0341-2040 184:4<229 2006 184 408 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer