caa a22 4500 467934983 CHVBK 20180406152954.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s00408-005-2589-5 doi (NATIONALLICENCE)springer-10.1007/s00408-005-2589-5 Release of Soluble E-Selectin from Activated Endothelial Cells upon Apoptosis [Elektronische Daten] [Elizabeth Harrington, Tihomir Stefanec, Julie Newton, Sharon Rounds] Circulating soluble E-selectin is increased in diseases associated with endothelial apoptosis such as sepsis and acute respiratory distress syndrome. We investigated the mechanism by which endothelial cell (EC) apoptosis may promote soluble E-selectin release. We found that serum deprivation of EC caused apoptosis, yet it did not induce E-selectin EC surface expression. Tumor necrosis factor-α (TNFα) significantly increased EC E-selectin surface expression. Soluble E-selectin was noted, however, only in the medium of TNFα-activated, apoptotic EC. Preincubation of the EC with the caspase inhibitor z-VAD-fmk significantly attenuated soluble E-selectin levels in the culture medium of TNFα-activated, apoptotic EC, but it had no effect on E-selectin surface expression. These results indicate that TNFα activation, but not apoptosis, is necessary for E-selectin surface expression in EC. Furthermore, E-selectin release from EC requires caspase-3 activation. Thus, increased concentrations of circulating E-selectin in serum may serve as a marker for endothelial apoptosis in certain disease states. Springer Science+Business Media, Inc., 2006 Apoptosis nationallicence Caspase nationallicence Endothelium nationallicence E-selectin nationallicence Harrington Elizabeth Vascular Research Laboratory, Providence Veterans Affairs Medical Center, Department of Medicine, Brown Medical School, Providence, Rhode Island aut Stefanec Tihomir Memorial Hospital, Pawtucket, Rhode Island aut Newton Julie Vascular Research Laboratory, Providence Veterans Affairs Medical Center, Department of Medicine, Brown Medical School, Providence, Rhode Island aut Rounds Sharon Vascular Research Laboratory, Providence Veterans Affairs Medical Center, Department of Medicine, Brown Medical School, Providence, Rhode Island aut Lung Springer-Verlag; www.springer-ny.com 184/5(2006-10-01), 259-266 0341-2040 184:5<259 2006 184 408 https://doi.org/10.1007/s00408-005-2589-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00408-005-2589-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Harrington Elizabeth Vascular Research Laboratory, Providence Veterans Affairs Medical Center, Department of Medicine, Brown Medical School, Providence, Rhode Island aut NATIONALLICENCE 700 1- Stefanec Tihomir Memorial Hospital, Pawtucket, Rhode Island aut NATIONALLICENCE 700 1- Newton Julie Vascular Research Laboratory, Providence Veterans Affairs Medical Center, Department of Medicine, Brown Medical School, Providence, Rhode Island aut NATIONALLICENCE 700 1- Rounds Sharon Vascular Research Laboratory, Providence Veterans Affairs Medical Center, Department of Medicine, Brown Medical School, Providence, Rhode Island aut NATIONALLICENCE 773 0- Lung Springer-Verlag; www.springer-ny.com 184/5(2006-10-01), 259-266 0341-2040 184:5<259 2006 184 408 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer