caa a22 4500 467935092 CHVBK 20180406152955.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s00408-005-2568-x doi (NATIONALLICENCE)springer-10.1007/s00408-005-2568-x TNF-α Promoter Polymorphism in Relation to TNF-α Production and Clinical Status in Cystic Fibrosis [Elektronische Daten] [Sabina Schmitt-Grohé, Frank Stüber, Malte Book, Joachim Bargon, Thomas Wagner, Christian Naujoks, Ralf Schubert, Michael Lentze, Stefan Zielen] The severity of lung disease in cystic fibrosis may be related to the genetic propensity of the host to produce tumor necrosis fector α (TNF-α). A polymorphism in the promoter region of the TNF-α gene at nucleotide 308 relative to the transcription start site may be important in determing the host's TNF-α response. The aim of this study was to assess the correlation between a TNF-308 promoter polymorphism, ex vivo TNF-α production (before and after lipopolysaccharide (LPS) stimulation), and clinical status [FEV1, weight (z-score), BMI, Shwachman score, incidence of diabetes mellitus, and Pseudomonas aeruginosa infection). Genotyping for the biallelic TNF-308 polymorphism was performed by using a real-time PCR cycler. Patients (homozygous for Delta F 508) were grouped according to genotype (TNF2 carriers, n = 16, median age = 15 yr, female/male = 5/11; TNF1 homozygotes, n = 37, median age = 21 yr, female/male = 18/19). TNF-α was measured using a chemiluminescent immunometric assay. There was a trend toward higher TNF-α values [median TNF2 carriers vs. TNF1 homozygotes: x = 56 vs. 43.5 pg/ml, n.s. (Mann-Whitney U-test] in those carrying the polymorphism and better lung function results [FEV1 (%) 81 vs. 65, n.s.]. These differences equalized [TNF2 carriers vs. TNF1 56 vs. 51 pg/ml, n.s.; FEV1 (%) 84 vs. 79, n.s.] after age adjustment (± 2 yr, n = 15, median age TNF2 vs. TNF1-17/18 yr). There were no significant differences for TNF values after LPS stimulation and the incidence of diabetes mellitus. The TNF-308 promoter polymorphism does not seem to influence TNF-α release in whole blood cells and clinical status. Springer Science+Business Media, Inc., 2006 Cystic fibrosis nationallicence TNF-α promoter polymorphism nationallicence TNF-α plasma levels nationallicence FEV1 nationallicence Diabetes mellitus nationallicence Schmitt-Grohé Sabina Departments of Pediatrics, University of Bonn, Bonn, Germanye aut Stüber Frank Department Anesthesiology, University of Bonn, Bonn, Germany aut Book Malte Department Anesthesiology, University of Bonn, Bonn, Germany aut Bargon Joachim Department of Pulmonary, University Hospital of Frankfurt, Frankfurt, Germany aut Wagner Thomas Department of Pulmonary, University Hospital of Frankfurt, Frankfurt, Germany aut Naujoks Christian Departments of Pediatrics, University of Bonn, Bonn, Germanye aut Schubert Ralf Department of Pediatric Pulmonary, University Hospital of Frankfurt, Frankfurt, Germany aut Lentze Michael Departments of Pediatrics, University of Bonn, Bonn, Germanye aut Zielen Stefan Department of Pediatric Pulmonary, University Hospital of Frankfurt, Frankfurt, Germany aut Lung Springer-Verlag; www.springer-ny.com 184/2(2006-04-01), 99-104 0341-2040 184:2<99 2006 184 408 https://doi.org/10.1007/s00408-005-2568-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00408-005-2568-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schmitt-Grohé Sabina Departments of Pediatrics, University of Bonn, Bonn, Germanye aut NATIONALLICENCE 700 1- Stüber Frank Department Anesthesiology, University of Bonn, Bonn, Germany aut NATIONALLICENCE 700 1- Book Malte Department Anesthesiology, University of Bonn, Bonn, Germany aut NATIONALLICENCE 700 1- Bargon Joachim Department of Pulmonary, University Hospital of Frankfurt, Frankfurt, Germany aut NATIONALLICENCE 700 1- Wagner Thomas Department of Pulmonary, University Hospital of Frankfurt, Frankfurt, Germany aut NATIONALLICENCE 700 1- Naujoks Christian Departments of Pediatrics, University of Bonn, Bonn, Germanye aut NATIONALLICENCE 700 1- Schubert Ralf Department of Pediatric Pulmonary, University Hospital of Frankfurt, Frankfurt, Germany aut NATIONALLICENCE 700 1- Lentze Michael Departments of Pediatrics, University of Bonn, Bonn, Germanye aut NATIONALLICENCE 700 1- Zielen Stefan Department of Pediatric Pulmonary, University Hospital of Frankfurt, Frankfurt, Germany aut NATIONALLICENCE 773 0- Lung Springer-Verlag; www.springer-ny.com 184/2(2006-04-01), 99-104 0341-2040 184:2<99 2006 184 408 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer