caa a22 4500 46793858X CHVBK 20180406153005.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s10822-006-9075-x doi (NATIONALLICENCE)springer-10.1007/s10822-006-9075-x Milestones in electron crystallography [Elektronische Daten] [Ludovic Renault, Hui Chou, Po Chiu, Rena Hill, Xiangyan Zeng, Bryant Gipson, Zi Zhang, Anchi Cheng, Vinzenz Unger, Henning Stahlberg] Electron crystallography determines the structure of membrane embedded proteins in the two-dimensionally crystallized state by cryo-transmission electron microscopy imaging and computer structure reconstruction. Milestones on the path to the structure are high-level expression, purification of functional protein, reconstitution into two-dimensional lipid membrane crystals, high-resolution imaging, and structure determination by computer image processing. Here we review the current state of these methods. We also created an Internet information exchange platform for electron crystallography, where guidelines for imaging and data processing method are maintained. The server (http://2dx.org) provides the electron crystallography community with a central information exchange platform, which is structured in blog and Wiki form, allowing visitors to add comments or discussions. It currently offers a detailed step-by-step introduction to image processing with the MRC software program. The server is also a repository for the 2dx software package, a user-friendly image processing system for 2D membrane protein crystals. Springer Science+Business Media, LLC, 2006 2dx nationallicence Electron crystallography nationallicence Membrane proteins nationallicence Image processing nationallicence 2D crystallization nationallicence Electron microscopy nationallicence 2D : Two-dimensional nationallicence 3D : Three-dimensional nationallicence cmc : Critical micellar concentration nationallicence cryo-EM : Cryo-electron microscopy nationallicence GUI : Graphical user interface nationallicence NMR : Nuclear magnetic resonance nationallicence TEM : Transmission electron microscope nationallicence XRD : X-ray diffraction nationallicence Renault Ludovic Molecular and Cellular Biology, College of Biological Sciences, University of California at Davis, Briggs Hall, 1 Shields Ave., 95616, Davis, CA, USA aut Chou Hui Molecular and Cellular Biology, College of Biological Sciences, University of California at Davis, Briggs Hall, 1 Shields Ave., 95616, Davis, CA, USA aut Chiu Po Molecular and Cellular Biology, College of Biological Sciences, University of California at Davis, Briggs Hall, 1 Shields Ave., 95616, Davis, CA, USA aut Hill Rena Molecular and Cellular Biology, College of Biological Sciences, University of California at Davis, Briggs Hall, 1 Shields Ave., 95616, Davis, CA, USA aut Zeng Xiangyan Molecular and Cellular Biology, College of Biological Sciences, University of California at Davis, Briggs Hall, 1 Shields Ave., 95616, Davis, CA, USA aut Gipson Bryant Molecular and Cellular Biology, College of Biological Sciences, University of California at Davis, Briggs Hall, 1 Shields Ave., 95616, Davis, CA, USA aut Zhang Zi Molecular and Cellular Biology, College of Biological Sciences, University of California at Davis, Briggs Hall, 1 Shields Ave., 95616, Davis, CA, USA aut Cheng Anchi Department of Cell Biology, The Scripps Research Institute, 1055 N. Torrey Pines Road, 02037, La Jolla, CA, USA aut Unger Vinzenz Department of Molecular Biophysics and Biochemistry, Yale University, P. O. Box 208024, 065200-8024, New Haven, CT, USA aut Stahlberg Henning Molecular and Cellular Biology, College of Biological Sciences, University of California at Davis, Briggs Hall, 1 Shields Ave., 95616, Davis, CA, USA aut Journal of Computer-Aided Molecular Design Kluwer Academic Publishers 20/7-8(2006-08-01), 519-527 0920-654X 20:7-8<519 2006 20 10822 https://doi.org/10.1007/s10822-006-9075-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10822-006-9075-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Renault Ludovic Molecular and Cellular Biology, College of Biological Sciences, University of California at Davis, Briggs Hall, 1 Shields Ave., 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Chou Hui Molecular and Cellular Biology, College of Biological Sciences, University of California at Davis, Briggs Hall, 1 Shields Ave., 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Chiu Po Molecular and Cellular Biology, College of Biological Sciences, University of California at Davis, Briggs Hall, 1 Shields Ave., 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Hill Rena Molecular and Cellular Biology, College of Biological Sciences, University of California at Davis, Briggs Hall, 1 Shields Ave., 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Zeng Xiangyan Molecular and Cellular Biology, College of Biological Sciences, University of California at Davis, Briggs Hall, 1 Shields Ave., 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Gipson Bryant Molecular and Cellular Biology, College of Biological Sciences, University of California at Davis, Briggs Hall, 1 Shields Ave., 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Zhang Zi Molecular and Cellular Biology, College of Biological Sciences, University of California at Davis, Briggs Hall, 1 Shields Ave., 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Cheng Anchi Department of Cell Biology, The Scripps Research Institute, 1055 N. Torrey Pines Road, 02037, La Jolla, CA, USA aut NATIONALLICENCE 700 1- Unger Vinzenz Department of Molecular Biophysics and Biochemistry, Yale University, P. O. Box 208024, 065200-8024, New Haven, CT, USA aut NATIONALLICENCE 700 1- Stahlberg Henning Molecular and Cellular Biology, College of Biological Sciences, University of California at Davis, Briggs Hall, 1 Shields Ave., 95616, Davis, CA, USA aut NATIONALLICENCE 773 0- Journal of Computer-Aided Molecular Design Kluwer Academic Publishers 20/7-8(2006-08-01), 519-527 0920-654X 20:7-8<519 2006 20 10822 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer