caa a22 4500 46794007X CHVBK 20180406153009.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s00421-006-0268-8 doi (NATIONALLICENCE)springer-10.1007/s00421-006-0268-8 Effects of endurance training on intracellular calcium concentration in T lymphocytes [Elektronische Daten] [Suzanne Broadbent, Gregory Gass] The purpose of the present study was to determine whether 12months of endurance training reduced [Ca2+]i in T helper (CD4+) lymphocytes in trained (TR) men compared to untrained (UT). Fourteen trained (Ironman triathletes) and nine untrained (sedentary) men volunteered for the study. The TR group averaged 12km of swimming, 300km of cycling and 60km of running per week during the year. Resting blood samples were taken from TR (VO2peak 64±2mlkg−1min−1) and UT (VO2peak 42±2mlkg−1min−1) subjects every 4weeks for 52weeks (October 1, 1999-October 1, 2000). Leukocyte concentration was measured using a full blood count. Unstimulated CD4+ lymphocytes were separated and analysed for changes in free ([Ca2+]i) and total ([Ca2+]t) calcium using flow cytometry. There were no significant differences in leukocyte concentration between UT and TR groups. There were significant differences between TR and UT in [Ca2+]i (October B and November), and [Ca2+]t (January and March). There were also significant sequential monthly changes in both [Ca2+]i and [Ca2+]t for TR and UT groups during the study. Significant increases in [Ca2+]i and [Ca2+]t during summer (January and March) for both TR and UT groups suggest an increase in intracellular signalling during hot weather. [Ca2+]i and [Ca2+]t were significantly lower in TR lymphocytes during November and March, suggesting that endurance training during warmer months may decrease [Ca2+]i through altered intracellular signalling, possibly to maintain lymphocyte function during heat stress. Springer-Verlag, 2006 Immunology nationallicence Lymphocyte proliferation nationallicence Signal transduction nationallicence [Ca2+]i nationallicence Broadbent Suzanne Institute of Food, Nutrition and Human Health, Massey University, Private Bag 756, Wellington, New Zealand aut Gass Gregory School of Physiotherapy and Exercise Science Griffith University, Gold Coast Campus, PMB 50 Gold Coast Mail Centre, 9726, Queensland, Australia aut European Journal of Applied Physiology Springer-Verlag 98/3(2006-10-01), 242-249 1439-6319 98:3<242 2006 98 421 https://doi.org/10.1007/s00421-006-0268-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00421-006-0268-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Broadbent Suzanne Institute of Food, Nutrition and Human Health, Massey University, Private Bag 756, Wellington, New Zealand aut NATIONALLICENCE 700 1- Gass Gregory School of Physiotherapy and Exercise Science Griffith University, Gold Coast Campus, PMB 50 Gold Coast Mail Centre, 9726, Queensland, Australia aut NATIONALLICENCE 773 0- European Journal of Applied Physiology Springer-Verlag 98/3(2006-10-01), 242-249 1439-6319 98:3<242 2006 98 421 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer