caa a22 4500 467940134 CHVBK 20180406153009.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s00421-006-0269-7 doi (NATIONALLICENCE)springer-10.1007/s00421-006-0269-7 Role of Hsp72 and norepinephrine in the moderate exercise-induced stimulation of neutrophils' microbicide capacity [Elektronische Daten] [E. Ortega, E. Giraldo, M. Hinchado, M. Martínez, S. Ibáñez, A. Cidoncha, M. Collazos, J. García] The influence of a single session of moderate exercise (45min at 55% of VO2 max) performed by young sedentary men (23-25years old) on the microbicidal capacity of neutrophils was compared by using both direct (killing of phagocytosed Candida albicans) and indirect (superoxide anion production measured by NBT reduction) techniques. In addition, the role of norepinephrine and heat shock protein (Hsp) 72 in the modulation of microbicide capacity of neutrophils was evaluated during the protocol of exercise and recovery period (24h). No significant changes were found in the superoxide production after exercise. However, immediately after exercise there was an increase in the destruction of C. albicans, which remained higher than basal values 1day later. This behaviour was similar to the changes found in the serum extracellular Hsp72 concentrations (an increase after exercise that remained higher than basal values 24h later). In vitro, the raised physiological concentration of Hsp72 after exercise also increased the microbicide capacity of neutrophils with respect to controls and the values induced by the basal concentration of the protein. This indicates that Hsp72 is participating as a "stress mediator” of the stimulated microbicide activity during moderate exercise. However, norepinephrine is not mediating the increased killing of C. albicans during exercise. Springer-Verlag, 2006 Exercise nationallicence Stress nationallicence Immunology nationallicence Neutrophils nationallicence Hsp72 nationallicence Norepinephrine nationallicence Ortega E. Department of Physiology, Faculty of Sciences, University of Extremadura, 06071, Badajoz, Spain aut Giraldo E. Department of Physiology, Faculty of Sciences, University of Extremadura, 06071, Badajoz, Spain aut Hinchado M. Department of Physiology, Faculty of Sciences, University of Extremadura, 06071, Badajoz, Spain aut Martínez M. Department of Physiology, Faculty of Sciences, University of Extremadura, 06071, Badajoz, Spain aut Ibáñez S. Faculty of Sport Sciences, University of Extremadura, Cáceres, Spain aut Cidoncha A. Hospital of D. Benito-Villanueva, Badajoz, Spain aut Collazos M. Department of Physiology, Faculty of Sciences, University of Extremadura, 06071, Badajoz, Spain aut García J. Department of Physiology, Faculty of Sciences, University of Extremadura, 06071, Badajoz, Spain aut European Journal of Applied Physiology Springer-Verlag 98/3(2006-10-01), 250-255 1439-6319 98:3<250 2006 98 421 https://doi.org/10.1007/s00421-006-0269-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00421-006-0269-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ortega E. Department of Physiology, Faculty of Sciences, University of Extremadura, 06071, Badajoz, Spain aut NATIONALLICENCE 700 1- Giraldo E. Department of Physiology, Faculty of Sciences, University of Extremadura, 06071, Badajoz, Spain aut NATIONALLICENCE 700 1- Hinchado M. Department of Physiology, Faculty of Sciences, University of Extremadura, 06071, Badajoz, Spain aut NATIONALLICENCE 700 1- Martínez M. Department of Physiology, Faculty of Sciences, University of Extremadura, 06071, Badajoz, Spain aut NATIONALLICENCE 700 1- Ibáñez S. Faculty of Sport Sciences, University of Extremadura, Cáceres, Spain aut NATIONALLICENCE 700 1- Cidoncha A. Hospital of D. Benito-Villanueva, Badajoz, Spain aut NATIONALLICENCE 700 1- Collazos M. Department of Physiology, Faculty of Sciences, University of Extremadura, 06071, Badajoz, Spain aut NATIONALLICENCE 700 1- García J. Department of Physiology, Faculty of Sciences, University of Extremadura, 06071, Badajoz, Spain aut NATIONALLICENCE 773 0- European Journal of Applied Physiology Springer-Verlag 98/3(2006-10-01), 250-255 1439-6319 98:3<250 2006 98 421 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer