caa a22 4500 467940223 CHVBK 20180406153010.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s00421-006-0279-5 doi (NATIONALLICENCE)springer-10.1007/s00421-006-0279-5 Intramyocellular lipid stores increase markedly in athletes after 1.5days lipid supplementation and are utilized during exercise in proportion to their content [Elektronische Daten] [Monica Zehnder, Emanuel Christ, Michael Ith, Kevin Acheson, Etienne Pouteau, Roland Kreis, Roman Trepp, Peter Diem, Chris Boesch, Jacques Décombaz] Intramyocellular lipids (IMCL) and muscle glycogen provide local energy during exercise (EX). The objective of this study was to clarify the role of high versus low IMCL levels at equal initial muscle glycogen on fuel selection during EX. After 3h of depleting exercise, 11 endurance-trained males consumed in a crossover design a high-carbohydrate (7gkg−1day−1) low-fat (0.5gkg−1day−1) diet (HC) for 2.5days or the same diet with 3gkg−1day−1 more fat provided during the last 1.5days of diet (four meals; HCF). Respiratory exchange, thigh muscle substrate breakdown by magnetic resonance spectroscopy, and plasma FFA oxidation ([1-13C]palmitate) were measured during EX (3h, 50% W max). Pre-EX IMCL concentrations were 55% higher after HCF. IMCL utilization during EX in HCF was threefold greater compared with HC (P<0.001) and was correlated with aerobic power and highly correlated (P<0.001) with initial content. Glycogen values and decrements during EX were similar. Whole-body fat oxidation (Fatox) was similar overall and plasma FFA oxidation smaller (P<0.05) during the first EX hour after HCF. Myocellular fuels contributed 8% more to whole-body energy demands after HCF (P<0.05) due to IMCL breakdown (27% Fatox). After EX, when both IMCL and glycogen concentrations were again similar across trials, a simulated 20-km time-trial showed no difference in performance between diets. In conclusion, IMCL concentrations can be increased during a glycogen loading diet by consuming additional fat for the last 1.5days. During subsequent exercise, IMCL decrease in proportion to their initial content, partly in exchange for peripheral fatty acids. Springer-Verlag, 2006 High-carbohydrate diet nationallicence Fat supplement nationallicence Muscle glycogen nationallicence Magnetic resonance spectroscopy nationallicence Zehnder Monica Department of Clinical Research, University and Inselspital, 3010, Bern, Switzerland aut Christ Emanuel Department of Endocrinology and Diabetes, Inselspital, 3010, Bern, Switzerland aut Ith Michael Department of Clinical Research, University and Inselspital, 3010, Bern, Switzerland aut Acheson Kevin Nestlé Research Center, PO Box 44, 1000, Lausanne 26, Switzerland aut Pouteau Etienne Nestlé Research Center, PO Box 44, 1000, Lausanne 26, Switzerland aut Kreis Roland Department of Clinical Research, University and Inselspital, 3010, Bern, Switzerland aut Trepp Roman Department of Endocrinology and Diabetes, Inselspital, 3010, Bern, Switzerland aut Diem Peter Department of Endocrinology and Diabetes, Inselspital, 3010, Bern, Switzerland aut Boesch Chris Department of Clinical Research, University and Inselspital, 3010, Bern, Switzerland aut Décombaz Jacques Nestlé Research Center, PO Box 44, 1000, Lausanne 26, Switzerland aut European Journal of Applied Physiology Springer-Verlag 98/4(2006-11-01), 341-354 1439-6319 98:4<341 2006 98 421 https://doi.org/10.1007/s00421-006-0279-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00421-006-0279-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zehnder Monica Department of Clinical Research, University and Inselspital, 3010, Bern, Switzerland aut NATIONALLICENCE 700 1- Christ Emanuel Department of Endocrinology and Diabetes, Inselspital, 3010, Bern, Switzerland aut NATIONALLICENCE 700 1- Ith Michael Department of Clinical Research, University and Inselspital, 3010, Bern, Switzerland aut NATIONALLICENCE 700 1- Acheson Kevin Nestlé Research Center, PO Box 44, 1000, Lausanne 26, Switzerland aut NATIONALLICENCE 700 1- Pouteau Etienne Nestlé Research Center, PO Box 44, 1000, Lausanne 26, Switzerland aut NATIONALLICENCE 700 1- Kreis Roland Department of Clinical Research, University and Inselspital, 3010, Bern, Switzerland aut NATIONALLICENCE 700 1- Trepp Roman Department of Endocrinology and Diabetes, Inselspital, 3010, Bern, Switzerland aut NATIONALLICENCE 700 1- Diem Peter Department of Endocrinology and Diabetes, Inselspital, 3010, Bern, Switzerland aut NATIONALLICENCE 700 1- Boesch Chris Department of Clinical Research, University and Inselspital, 3010, Bern, Switzerland aut NATIONALLICENCE 700 1- Décombaz Jacques Nestlé Research Center, PO Box 44, 1000, Lausanne 26, Switzerland aut NATIONALLICENCE 773 0- European Journal of Applied Physiology Springer-Verlag 98/4(2006-11-01), 341-354 1439-6319 98:4<341 2006 98 421 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer