caa a22 4500 467940428 CHVBK 20180406153010.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s00421-006-0227-4 doi (NATIONALLICENCE)springer-10.1007/s00421-006-0227-4 Prolonged exercise does not cause lymphocyte DNA damage or increased apoptosis in well-trained endurance athletes [Elektronische Daten] [E. Peters, M. Van Eden, N. Tyler, A. Ramautar, A. Chuturgoon] Recent research has demonstrated that lymphocyte apoptosis sensitivity appears to be related to training status and exercise intensity. This work investigated the effect of prolonged, submaximal treadmill running on percentage (%) apoptosis, % necrosis and DNA strand breaks in lymphocytes and related these to changes in total lymphocyte and blood cortisol concentrations in well-trained runners. Venous blood samples (n=14) were taken immediately before (PRE), immediately after (IPE) and 3h after (3PE) 2.5h of treadmill running at 75% of VO2 max from eight well-trained male endurance athletes (age 34.2±2.44years) and analysed for cellular content and serum cortisol concentrations. Lymphocytes were isolated from whole blood and % apoptotic and necrotic cell were detected by flow cytometry using Annexin V-FITC and propidium iodide uptake. DNA strand breaks were measured by single-cell gel electrophoresis. Despite a significant (P<0.001) exercise-induced increase in mean serum cortisol concentrations and reduction in lymphocyte counts, the mean % Annexin-V positive cells (13.3±6.78 in PRE, 11.3±5.51 in IPE and 12.8±6.75 in 3PE samples) were not significantly different at the three time-points (P>0.05). Mean DNA strand breaks in the lymphocytes also did not change significantly (P>0.05) rising from 25.7±2.16 to 26.9±1.89 and 27.1±1.38μm in IPE and 3PE samples, respectively. The exercise-induced changes in total blood lymphocyte counts and cortisol concentrations did not result in a significant change in % apoptotic lymphocytes or DNA strand breaks in the endurance-trained athletes during this prolonged, submaximal exercise. Springer-Verlag, 2006 Lymphocytopenia nationallicence Cortisol nationallicence DNA migration nationallicence Annexin-FITC nationallicence Peters E. School of Medical Sciences, Faculty of Health Sciences, University of KwaZulu-Natal, 519, Umbilo Road, Congella 4013, Private Bag 7, 4001, Durban, South Africa aut Van Eden M. School of Medical Sciences, Faculty of Health Sciences, University of KwaZulu-Natal, 519, Umbilo Road, Congella 4013, Private Bag 7, 4001, Durban, South Africa aut Tyler N. School of Medical Sciences, Faculty of Health Sciences, University of KwaZulu-Natal, 519, Umbilo Road, Congella 4013, Private Bag 7, 4001, Durban, South Africa aut Ramautar A. School of Medical Sciences, Faculty of Health Sciences, University of KwaZulu-Natal, 519, Umbilo Road, Congella 4013, Private Bag 7, 4001, Durban, South Africa aut Chuturgoon A. School of Medical Sciences, Faculty of Health Sciences, University of KwaZulu-Natal, 519, Umbilo Road, Congella 4013, Private Bag 7, 4001, Durban, South Africa aut European Journal of Applied Physiology Springer-Verlag 98/2(2006-09-01), 124-131 1439-6319 98:2<124 2006 98 421 https://doi.org/10.1007/s00421-006-0227-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00421-006-0227-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Peters E. School of Medical Sciences, Faculty of Health Sciences, University of KwaZulu-Natal, 519, Umbilo Road, Congella 4013, Private Bag 7, 4001, Durban, South Africa aut NATIONALLICENCE 700 1- Van Eden M. School of Medical Sciences, Faculty of Health Sciences, University of KwaZulu-Natal, 519, Umbilo Road, Congella 4013, Private Bag 7, 4001, Durban, South Africa aut NATIONALLICENCE 700 1- Tyler N. School of Medical Sciences, Faculty of Health Sciences, University of KwaZulu-Natal, 519, Umbilo Road, Congella 4013, Private Bag 7, 4001, Durban, South Africa aut NATIONALLICENCE 700 1- Ramautar A. School of Medical Sciences, Faculty of Health Sciences, University of KwaZulu-Natal, 519, Umbilo Road, Congella 4013, Private Bag 7, 4001, Durban, South Africa aut NATIONALLICENCE 700 1- Chuturgoon A. School of Medical Sciences, Faculty of Health Sciences, University of KwaZulu-Natal, 519, Umbilo Road, Congella 4013, Private Bag 7, 4001, Durban, South Africa aut NATIONALLICENCE 773 0- European Journal of Applied Physiology Springer-Verlag 98/2(2006-09-01), 124-131 1439-6319 98:2<124 2006 98 421 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer