caa a22 4500 46794072X CHVBK 20180406153011.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s00421-005-0100-x doi (NATIONALLICENCE)springer-10.1007/s00421-005-0100-x The effects of short-term sprint training on MCT expression in moderately endurance-trained runners [Elektronische Daten] [Dale Bickham, David Bentley, Peter Rossignol, David Cameron-Smith] The purpose of this study was to assess the effects of short-term sprint training on transient changes in monocarboxylate lactate transporter 1 (MCT1) and MCT4 protein and mRNA content. Seven moderately endurance-trained runners (mean±SE; age 27.7±2.9years, body mass 81.1±5.9kg, $$ \ifmmode\expandafter\dot\else\expandafter\.\fi{V}{\text{O}}_{2} \;\max $$ 58.1±2.0mlkg−1min−1) completed a $$ \ifmmode\expandafter\dot\else\expandafter\.\fi{V}{\text{O}}_{2} \;\max $$ and a supramaximal running test to exhaustion (RTE) before and after a 6-week period of sprint training. The sprint training was progressive and consisted of 18 sessions of near maximal short duration (5-15s) sprints to compliment the athlete's endurance training. Prior to the training period there was a significant (P<0.05) increase in MCT1, but not MCT4 protein, 2h after the RTE. This occurred without any change in corresponding mRNA levels. After the training period, there was a significant increase in MCT1 protein but no significant change in the MCT4 isoform. Both MCT1 and MCT4 mRNA was significantly lower at rest and 2h post-RTE after the completion of the training period. After the training period, there was a significant increase in the time to exhaustion and distance covered during the RTE. This study demonstrates that sprint training of this length and type results in an upregulation of MCT1 protein, but not MCT4 content. Additionally, this study shows conflicting adaptations in MCT1 and MCT4 protein and mRNA levels following training, which may indicate post-transcriptional regulation of MCT expression in human muscle. Springer-Verlag, 2005 Anaerobic nationallicence Running nationallicence Fiber type nationallicence Interval nationallicence Lactate transport nationallicence Bickham Dale School of Nutrition and Exercise Sciences, Deakin University, Melbourne, VIC, Australia aut Bentley David Health and Sport Science, Department of Medical Science, University of New South Wales, Sydney, NSW, Australia aut Rossignol Peter Human Movement Studies, Queensland University of Technology, Brisbane, QLD, Australia aut Cameron-Smith David School of Nutrition and Exercise Sciences, Deakin University, Melbourne, VIC, Australia aut European Journal of Applied Physiology Springer-Verlag 96/6(2006-04-01), 636-643 1439-6319 96:6<636 2006 96 421 https://doi.org/10.1007/s00421-005-0100-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00421-005-0100-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bickham Dale School of Nutrition and Exercise Sciences, Deakin University, Melbourne, VIC, Australia aut NATIONALLICENCE 700 1- Bentley David Health and Sport Science, Department of Medical Science, University of New South Wales, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Rossignol Peter Human Movement Studies, Queensland University of Technology, Brisbane, QLD, Australia aut NATIONALLICENCE 700 1- Cameron-Smith David School of Nutrition and Exercise Sciences, Deakin University, Melbourne, VIC, Australia aut NATIONALLICENCE 773 0- European Journal of Applied Physiology Springer-Verlag 96/6(2006-04-01), 636-643 1439-6319 96:6<636 2006 96 421 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer