caa a22 4500 467940851 CHVBK 20180406153012.0 cr unu---uuuuu 170328e20060201xx s 000 0 eng 10.1007/s00421-005-0071-y doi (NATIONALLICENCE)springer-10.1007/s00421-005-0071-y Influence of cyclic mechanical strain and heat of human tendon fibroblasts on HSP-72 [Elektronische Daten] [M. Jagodzinski, S. Hankemeier, M. van Griensven, U. Bosch, C. Krettek, J. Zeichen] Heat shock protein 72 (HSP-72) is a member of a superfamily of different proteins that are synthesized as a cytoprotective response following cellular stress. Mechanical strain is an important component in ligament and tendon healing. Up to the present point of time, the influence of mechanical strain on the expression of HSP-72 is unknown. Tendon fibroblasts from the patellar tendons of nine individuals were isolated and amplified in vitro. First, the effect of 15 or 60min of heat exposition was studied immunohistochemically and by Western blotting. In a second experiment, the effects of 15 and 60min of cyclic longitudinal stretching were investigated. Samples were taken after 2, 4 and 8h. The heat exposition experiments indicate that HSP-72 accumulates in the nucleus and that there is a transient upregulation. This effect is more prominent after 60 min of heat exposure. The same reaction was found after stretching stimulation, however, to a lesser extent. There was a transient up regulation of HSP-72 after short-term stretching and a biphasic increase after 60min of stretching. Upregulation of HSP-72 by heat and mechanical stress is a response in human fibroblasts which involves a nuclear translocation. The response differs with regard to the time points beyond 2h after the application of either stress. Springer-Verlag, 2005 Mechanical nationallicence Strain nationallicence Stretching nationallicence Fibroblast nationallicence HSP-72 nationallicence Jagodzinski M. Department of Trauma Surgery, Hanover Medical School (MHH), Carl-Neuberg-Str. 1, 30625, Hanover, Germany aut Hankemeier S. Department of Trauma Surgery, Hanover Medical School (MHH), Carl-Neuberg-Str. 1, 30625, Hanover, Germany aut van Griensven M. Department of Trauma Surgery, Hanover Medical School (MHH), Carl-Neuberg-Str. 1, 30625, Hanover, Germany aut Bosch U. Center of Orthopaedic Surgery and Sports Traumatology, INI, Alexis-Carrel-Str. 4, 30625, Hanover, Germany aut Krettek C. Department of Trauma Surgery, Hanover Medical School (MHH), Carl-Neuberg-Str. 1, 30625, Hanover, Germany aut Zeichen J. Department of Trauma Surgery, Hanover Medical School (MHH), Carl-Neuberg-Str. 1, 30625, Hanover, Germany aut European Journal of Applied Physiology Springer-Verlag 96/3(2006-02-01), 249-256 1439-6319 96:3<249 2006 96 421 https://doi.org/10.1007/s00421-005-0071-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00421-005-0071-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jagodzinski M. Department of Trauma Surgery, Hanover Medical School (MHH), Carl-Neuberg-Str. 1, 30625, Hanover, Germany aut NATIONALLICENCE 700 1- Hankemeier S. Department of Trauma Surgery, Hanover Medical School (MHH), Carl-Neuberg-Str. 1, 30625, Hanover, Germany aut NATIONALLICENCE 700 1- van Griensven M. Department of Trauma Surgery, Hanover Medical School (MHH), Carl-Neuberg-Str. 1, 30625, Hanover, Germany aut NATIONALLICENCE 700 1- Bosch U. Center of Orthopaedic Surgery and Sports Traumatology, INI, Alexis-Carrel-Str. 4, 30625, Hanover, Germany aut NATIONALLICENCE 700 1- Krettek C. Department of Trauma Surgery, Hanover Medical School (MHH), Carl-Neuberg-Str. 1, 30625, Hanover, Germany aut NATIONALLICENCE 700 1- Zeichen J. Department of Trauma Surgery, Hanover Medical School (MHH), Carl-Neuberg-Str. 1, 30625, Hanover, Germany aut NATIONALLICENCE 773 0- European Journal of Applied Physiology Springer-Verlag 96/3(2006-02-01), 249-256 1439-6319 96:3<249 2006 96 421 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer