caa a22 4500 46794105X CHVBK 20180627064201.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s00421-005-0082-8 doi (NATIONALLICENCE)springer-10.1007/s00421-005-0082-8 Gene expression in skeletal muscle of coronary artery disease patients after concentric and eccentric endurance training [Elektronische Daten] [J. Zoll, R. Steiner, K. Meyer, M. Vogt, H. Hoppeler, M. Flück] Low-intensity concentric (CET) and eccentric (EET) endurance-type training induce specific structural adaptations in skeletal muscle. We evaluated to which extent steady-state adaptations in transcript levels are involved in the compensatory alterations of muscle mitochondria and myofibrils with CET versus EET at a matched metabolic exercise intensity of medicated, stable coronary patients (CAD). Biopsies were obtained from vastus lateralis muscle before and after 8weeks of CET (n=6) or EET (n=6). Transcript levels for factors involved in mitochondrial biogenesis (PGC-1α, Tfam), mitochondrial function (COX-1, COX-4), control of contractile phenotype (MyHC I, IIa, IIx) as well as mechanical stress marker (IGF-I) were quantified using an reverse-transcriptase polymerase chain reaction approach. After 8weeks of EET, a reduction of the COX-4mRNA level by 41% and a tendency for a drop in Tfam transcript concentration (−33%, P=0.06) was noted. This down-regulation corresponded to a drop in total mitochondrial volume density. MyHC-IIa transcript levels were specifically decreased after EET, and MyHC-I mRNA showed a trend towards a reduction (P=0.08). Total fiber cross-sectional area was not altered. After CET and EET, the IGF-I mRNA level was significantly increased. The PGC-1α significantly correlated with Tfam, and both PGC-1α and Tfam significantly correlated with COX-1 and COX-4mRNAs. Post-hoc analysis identified significant interactions between the concurrent medication and muscular transcript levels as well as fiber size. Our findings support the concept that specific transcriptional adaptations mediate the divergent mitochondrial response of muscle cells to endurance training under different load condition and indicate a mismatch of processes related to muscle hypertrophy in medicated CAD patients. Springer-Verlag, 2005 Muscle plasticity nationallicence Mechanical stress nationallicence Metabolic stress nationallicence mRNA nationallicence Eccentric exercise nationallicence CET : Concentric endurance-type training nationallicence EET : Eccentric endurance-type training nationallicence RT-PCR : Reverse-transcriptase polymerase chain reaction nationallicence Zoll J. Department of Anatomy, University of Bern, Baltzerstrasse 2, 3000, Bern 9, Switzerland aut Steiner R. Cardiovascular Prevention and Rehabilitation, Department of Cardiology, University Hospital, 3010, Bern, Switzerland aut Meyer K. Cardiovascular Prevention and Rehabilitation, Department of Cardiology, University Hospital, 3010, Bern, Switzerland aut Vogt M. Department of Anatomy, University of Bern, Baltzerstrasse 2, 3000, Bern 9, Switzerland aut Hoppeler H. Department of Anatomy, University of Bern, Baltzerstrasse 2, 3000, Bern 9, Switzerland aut Flück M. Department of Anatomy, University of Bern, Baltzerstrasse 2, 3000, Bern 9, Switzerland aut European Journal of Applied Physiology Springer-Verlag 96/4(2006-03-01), 413-422 1439-6319 96:4<413 2006 96 421 https://doi.org/10.1007/s00421-005-0082-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00421-005-0082-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zoll J. Department of Anatomy, University of Bern, Baltzerstrasse 2, 3000, Bern 9, Switzerland aut NATIONALLICENCE 700 1- Steiner R. Cardiovascular Prevention and Rehabilitation, Department of Cardiology, University Hospital, 3010, Bern, Switzerland aut NATIONALLICENCE 700 1- Meyer K. Cardiovascular Prevention and Rehabilitation, Department of Cardiology, University Hospital, 3010, Bern, Switzerland aut NATIONALLICENCE 700 1- Vogt M. Department of Anatomy, University of Bern, Baltzerstrasse 2, 3000, Bern 9, Switzerland aut NATIONALLICENCE 700 1- Hoppeler H. Department of Anatomy, University of Bern, Baltzerstrasse 2, 3000, Bern 9, Switzerland aut NATIONALLICENCE 700 1- Flück M. Department of Anatomy, University of Bern, Baltzerstrasse 2, 3000, Bern 9, Switzerland aut NATIONALLICENCE 773 0- European Journal of Applied Physiology Springer-Verlag 96/4(2006-03-01), 413-422 1439-6319 96:4<413 2006 96 421 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 46794105X BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer