caa a22 4500 467941653 CHVBK 20180406153014.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s00421-006-0146-4 doi (NATIONALLICENCE)springer-10.1007/s00421-006-0146-4 The effects of intensive, moderate and downhill treadmill running on human blood lymphocytes expressing the adhesion/activation molecules CD54 (ICAM-1), CD18 (β2 integrin) and CD53 [Elektronische Daten] [Richard Simpson, Geraint Florida-James, Greg Whyte, Keith Guy] This study examined the effects of intensive, moderate and downhill treadmill running on blood lymphocyte expression of adhesion/activation (AA) molecules. Trained subjects completed three treadmill-running protocols of identical duration: (1) an intensive protocol at 80% $$ \ifmmode\expandafter\dot\else\expandafter\.\fi{V}_{{{\text{O}}_{{2\max }} }} $$ to volitional exhaustion, (2) a moderate protocol at 60% $$ \ifmmode\expandafter\dot\else\expandafter\.\fi{V}_{{{\text{O}}_{{2\max }} }} $$ and (3) a −10% downhill (eccentric) protocol at 80% $$ \ifmmode\expandafter\dot\else\expandafter\.\fi{V}_{{{\text{O}}_{{2\max }} }} $$ . Blood samples were taken before, immediately after, 1 and 24h after exercise. Isolated lymphocytes were assessed for expression of the AA molecules CD54, CD18 and CD53 by flow cytometry. Lymphocyte counts increased immediately after all running protocols. Lymphocytopenia was observed 1h after the intensive and eccentric protocols only. Plasma creatine kinase increased 24h after the downhill protocol only. Increases in the number and percentage of CD54+, CD18bright and CD53bright lymphocytes were observed immediately after the intensive and eccentric protocols, with the numbers falling below pre-exercise values at 1h post-exercise for all protocols. No differences were found between the intensive protocol and the eccentric protocol at the same relative intensity. Analysis of lymphocyte subsets showed that the total number of CD3+, CD4+, CD8+ and CD56+ lymphocytes increased after the intensive protocol before falling below pre-exercise values at 1h post-exercise. A relatively greater mobilisation of CD56+ and CD8+ cells accounts for the changes in CD54+, CD18bright and CD53bright cell populations. Lymphocytes that enter and exit the circulation following exercise express high levels of AA molecules, which may mediate extravasation and post-exercise lymphocytopenia. This effect appears to be influenced by exercise intensity and not muscle damage. Springer-Verlag, 2006 Lymphocytosis nationallicence Lymphocytopenia nationallicence CD antigens nationallicence Muscle damage nationallicence Flow cytometry nationallicence Simpson Richard Biomedicine and Sport and Exercise Science Research Group, School of Life Sciences, Napier University, 10 Colinton Road, EH10 5DT, Edinburgh, Scotland, UK aut Florida-James Geraint Biomedicine and Sport and Exercise Science Research Group, School of Life Sciences, Napier University, 10 Colinton Road, EH10 5DT, Edinburgh, Scotland, UK aut Whyte Greg The Performance Centre, English Institute of Sport, Bisham Abbey NSC, SL7 1RT, Marlow, Bucks, UK aut Guy Keith Biomedicine and Sport and Exercise Science Research Group, School of Life Sciences, Napier University, 10 Colinton Road, EH10 5DT, Edinburgh, Scotland, UK aut European Journal of Applied Physiology Springer-Verlag 97/1(2006-05-01), 109-121 1439-6319 97:1<109 2006 97 421 https://doi.org/10.1007/s00421-006-0146-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00421-006-0146-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Simpson Richard Biomedicine and Sport and Exercise Science Research Group, School of Life Sciences, Napier University, 10 Colinton Road, EH10 5DT, Edinburgh, Scotland, UK aut NATIONALLICENCE 700 1- Florida-James Geraint Biomedicine and Sport and Exercise Science Research Group, School of Life Sciences, Napier University, 10 Colinton Road, EH10 5DT, Edinburgh, Scotland, UK aut NATIONALLICENCE 700 1- Whyte Greg The Performance Centre, English Institute of Sport, Bisham Abbey NSC, SL7 1RT, Marlow, Bucks, UK aut NATIONALLICENCE 700 1- Guy Keith Biomedicine and Sport and Exercise Science Research Group, School of Life Sciences, Napier University, 10 Colinton Road, EH10 5DT, Edinburgh, Scotland, UK aut NATIONALLICENCE 773 0- European Journal of Applied Physiology Springer-Verlag 97/1(2006-05-01), 109-121 1439-6319 97:1<109 2006 97 421 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer