caa a22 4500 46794170X CHVBK 20180406153014.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s00421-006-0193-x doi (NATIONALLICENCE)springer-10.1007/s00421-006-0193-x The effects of high-intensity exercise on skeletal muscle neutrophil myeloperoxidase in untrained and trained rats [Elektronische Daten] [Vladimir Morozov, Pavel Tsyplenkov, Natalia Golberg, Michael Kalinski] The primary purpose of this study was to examine the effects of high-intensity acute exercise on neutrophil infiltration in different muscle fiber types of untrained rats and to compare postexercise neutrophil accumulation in muscles of untrained and trained animals. The effect of high-intensity acute exercise on blood neutrophil degranulation reaction in trained animals was also elucidated. Neutrophil enzyme myeloperoxidase (MPO) was determined as a measure of neutrophil migration into muscles and blood neutrophil degranulation. Male albino rats were subjected to acute exercise and 5weeks of training. The used model of intensive acute exercise consisted of 5, 15, and 25 intermittent swimming bouts with the addition of weight (8% of total body mass) for 1-min each, followed by 1.5-min rest intervals. MPO was analyzed in quadriceps muscle (white and red portion) and in soleus muscle 24h after acute exercise. MPO content in resting blood plasma and neutrophils was determined 48-h following the completion of a training process. In addition, MPO content in the trained rats was measured immediately (in blood plasma and neutrophils) after and 24h (in muscles) following a single-bout of exercise to exhaustion. The remaining two-third of the trained animals were exposed to a single-bout of nonstop swimming with the addition of 6% body mass until exhaustion. These animals were sacrificed immediately and 24h after loaded swimming to analyze leukocyte count, MPO content in blood plasma and neutrophils and in muscles, respectively. About 24h after exercise MPO concentrations in the red portion of quadriceps muscle and in soleus muscle were 4-7-fold higher as compared to the white portion of m. quadriceps. There was an association between the quantity of repetitive bouts of swimming and MPO content in the muscles. The duration of swimming to exhaustion of trained rats was 3.8-fold longer than untrained sedentary control. At rest, plasma MPO concentration was found to be 40% higher in trained rats compared to untrained controls (P<0.05). Postexercise plasma MPO concentrations were significantly higher both in untrained (+137%; P<0.05) and trained (+81%; P<0.05) rats compared to resting values. At rest neutrophil MPO concentration was found to be 33% lower in trained rats compared to untrained controls (P<0.05). There were no significant differences in muscle MPO concentrations between untrained and trained rats at rest. A single-bout of exercise to exhaustion produced a greater increase in MPO content in untrained compared to trained rats. The data suggest that postexercise neutrophil infiltration is more intensive in red fibers types compared to white fiber types. A smaller neutrophil infiltration in muscles of trained animals after exhaustive exercise suggests a protective effect of previous training to muscle injury. Springer-Verlag, 2006 Exercise nationallicence Training nationallicence Skeletal muscle nationallicence Neutrophil nationallicence Myeloperoxidase nationallicence Morozov Vladimir Department of Biochemistry, Research Institute of Physical Culture, Dynamo Ave. 2, 197110, St. Petersburg, Russia aut Tsyplenkov Pavel Department of Biochemistry, Research Institute of Physical Culture, Dynamo Ave. 2, 197110, St. Petersburg, Russia aut Golberg Natalia Department of Biochemistry, Research Institute of Physical Culture, Dynamo Ave. 2, 197110, St. Petersburg, Russia aut Kalinski Michael Exercise Physiology Laboratory, School of Exercise, Leisure and Sport, College and Graduate School of Education, Health and Human Services, Kent State University, 44242-0001, Kent, OH, USA aut European Journal of Applied Physiology Springer-Verlag 97/6(2006-08-01), 716-722 1439-6319 97:6<716 2006 97 421 https://doi.org/10.1007/s00421-006-0193-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00421-006-0193-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Morozov Vladimir Department of Biochemistry, Research Institute of Physical Culture, Dynamo Ave. 2, 197110, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Tsyplenkov Pavel Department of Biochemistry, Research Institute of Physical Culture, Dynamo Ave. 2, 197110, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Golberg Natalia Department of Biochemistry, Research Institute of Physical Culture, Dynamo Ave. 2, 197110, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Kalinski Michael Exercise Physiology Laboratory, School of Exercise, Leisure and Sport, College and Graduate School of Education, Health and Human Services, Kent State University, 44242-0001, Kent, OH, USA aut NATIONALLICENCE 773 0- European Journal of Applied Physiology Springer-Verlag 97/6(2006-08-01), 716-722 1439-6319 97:6<716 2006 97 421 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer