caa a22 4500 467941793 CHVBK 20180406153015.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s00421-006-0225-6 doi (NATIONALLICENCE)springer-10.1007/s00421-006-0225-6 Quantitative analysis of muscle hardness in tetanic contractions induced by electrical stimulation in rats [Elektronische Daten] [Makoto Morisada, Kaoru Okada, Kenji Kawakita] The purpose of this study was to investigate the in vivo relation between muscle hardness during an electrically induced contracting state and neuromuscular functions (M-wave and developed tension). Sixteen Sprague-Dawley rats were deeply anesthetized with urethane. Muscle hardness was measured quantitatively at the mid-portion of the gastrocnemius (GS) muscle during tetanic contractions induced by electrical stimulation (50Hz, 100μs duration) of the sciatic nerve or of the muscle directly. The M-wave was recorded with a pair of wire electrodes inserted into the muscle, and the developed tension was monitored with a push-pull gauge. Muscle hardness, M-wave amplitude and developed tension increased rapidly with the onset of nerve stimulation. Similar but intensity-dependent increases in muscle hardness and tension were observed following direct tetanic stimulation of the muscle. The hardness measured during nerve stimulation was correlated with the amplitude of the M-wave (r=0.62, P<0.0001) and the developed tension (r=0.85, P<0.0001). These phenomena were suppressed by pancuronium treatment (2mg/ml, i.v.). These results suggest that muscle tension might be the most important factor for transcutaneously measured muscle hardness induced by tetanic muscle contraction. Springer-Verlag, 2006 Muscle hardness nationallicence M-wave nationallicence Muscle tension nationallicence Electrical stimulation nationallicence Pancuronium nationallicence Morisada Makoto Department of Physiology, Meiji University of Oriental Medicine, Hiyoshi-cho, Nantan-city, 629-0392, Kyoto, Japan aut Okada Kaoru Department of Physiology, Meiji University of Oriental Medicine, Hiyoshi-cho, Nantan-city, 629-0392, Kyoto, Japan aut Kawakita Kenji Department of Physiology, Meiji University of Oriental Medicine, Hiyoshi-cho, Nantan-city, 629-0392, Kyoto, Japan aut European Journal of Applied Physiology Springer-Verlag 97/6(2006-08-01), 681-686 1439-6319 97:6<681 2006 97 421 https://doi.org/10.1007/s00421-006-0225-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00421-006-0225-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Morisada Makoto Department of Physiology, Meiji University of Oriental Medicine, Hiyoshi-cho, Nantan-city, 629-0392, Kyoto, Japan aut NATIONALLICENCE 700 1- Okada Kaoru Department of Physiology, Meiji University of Oriental Medicine, Hiyoshi-cho, Nantan-city, 629-0392, Kyoto, Japan aut NATIONALLICENCE 700 1- Kawakita Kenji Department of Physiology, Meiji University of Oriental Medicine, Hiyoshi-cho, Nantan-city, 629-0392, Kyoto, Japan aut NATIONALLICENCE 773 0- European Journal of Applied Physiology Springer-Verlag 97/6(2006-08-01), 681-686 1439-6319 97:6<681 2006 97 421 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer