caa a22 4500 467941904 CHVBK 20180406153015.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s00421-006-0197-6 doi (NATIONALLICENCE)springer-10.1007/s00421-006-0197-6 Skeletal muscle atrophy increases cell proliferation in mice gastrocnemius during the first week of hindlimb suspension [Elektronische Daten] [Rita Ferreira, Maria Neuparth, António Ascensão, José Magalhães, Rui Vitorino, José Duarte, Francisco Amado] The comprehension of the cellular mechanisms underlying skeletal muscle atrophy has been the aim of several experimental studies. However, the majority of them focused on alterations of the myocytes induced by different experimental conditions yet disregarding the contribution of other cells such as endothelial cells and fibroblasts. In this sense, 70 Charles River CD1 male mice were randomly assigned to seven groups (n=10 per group): control and 6, 12, 24, 48, 72h and 1week with respect to the period of hindlimb suspension. Forty-eight hours before sacrifice, the animals were injected with bromodeoxyuridine (BrdU) in order to identify proliferating cells. Immunohistochemistry and south-western blotting techniques were used to evaluate across the whole gastrocnemius muscle BrdU incorporation into the different proliferating cells. The contribution of the apoptotic response was also measured in order to ascertain whether the balance between cell survival and death was preserved. The results observed during 1week of unloading-induced atrophy evidenced an intense peak of proliferating activity only after 6h, mainly due to the duplication of satellite cells. Consequently to this unexpected activation of satellite cells, the addition of nuclei to the fibre syncytium was recognized at 12h of unloading. After 48h of weightlessness, the proliferating activity observed was largely due to an interstitial fibrosis. According to the apoptotic index profile observed during the analysed unloading period, this general proliferative activity was balanced by apoptosis, which strongly suggests the existence of a regulatory feedback response between anabolic and catabolic events in unloading-induced skeletal muscle atrophy. Springer-Verlag, 2006 Skeletal muscle atrophy nationallicence Hindlimb suspension nationallicence Cell proliferation nationallicence Apoptosis nationallicence Satellite cells nationallicence Fibroblasts nationallicence Endothelial cells nationallicence Ferreira Rita CIAFEL, Laboratory of Sport Biochemistry, Faculty of Sports Sciences, University of Porto, Rua Dr. Plácido Costa, 91, 4200, Porto, Portugal aut Neuparth Maria CIAFEL, Laboratory of Sport Biochemistry, Faculty of Sports Sciences, University of Porto, Rua Dr. Plácido Costa, 91, 4200, Porto, Portugal aut Ascensão António CIAFEL, Laboratory of Sport Biochemistry, Faculty of Sports Sciences, University of Porto, Rua Dr. Plácido Costa, 91, 4200, Porto, Portugal aut Magalhães José CIAFEL, Laboratory of Sport Biochemistry, Faculty of Sports Sciences, University of Porto, Rua Dr. Plácido Costa, 91, 4200, Porto, Portugal aut Vitorino Rui CIAFEL, Laboratory of Sport Biochemistry, Faculty of Sports Sciences, University of Porto, Rua Dr. Plácido Costa, 91, 4200, Porto, Portugal aut Duarte José CIAFEL, Laboratory of Sport Biochemistry, Faculty of Sports Sciences, University of Porto, Rua Dr. Plácido Costa, 91, 4200, Porto, Portugal aut Amado Francisco Chemistry Department, University of Aveiro, Porto, Portugal aut European Journal of Applied Physiology Springer-Verlag 97/3(2006-06-01), 340-346 1439-6319 97:3<340 2006 97 421 https://doi.org/10.1007/s00421-006-0197-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00421-006-0197-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ferreira Rita CIAFEL, Laboratory of Sport Biochemistry, Faculty of Sports Sciences, University of Porto, Rua Dr. Plácido Costa, 91, 4200, Porto, Portugal aut NATIONALLICENCE 700 1- Neuparth Maria CIAFEL, Laboratory of Sport Biochemistry, Faculty of Sports Sciences, University of Porto, Rua Dr. Plácido Costa, 91, 4200, Porto, Portugal aut NATIONALLICENCE 700 1- Ascensão António CIAFEL, Laboratory of Sport Biochemistry, Faculty of Sports Sciences, University of Porto, Rua Dr. Plácido Costa, 91, 4200, Porto, Portugal aut NATIONALLICENCE 700 1- Magalhães José CIAFEL, Laboratory of Sport Biochemistry, Faculty of Sports Sciences, University of Porto, Rua Dr. Plácido Costa, 91, 4200, Porto, Portugal aut NATIONALLICENCE 700 1- Vitorino Rui CIAFEL, Laboratory of Sport Biochemistry, Faculty of Sports Sciences, University of Porto, Rua Dr. Plácido Costa, 91, 4200, Porto, Portugal aut NATIONALLICENCE 700 1- Duarte José CIAFEL, Laboratory of Sport Biochemistry, Faculty of Sports Sciences, University of Porto, Rua Dr. Plácido Costa, 91, 4200, Porto, Portugal aut NATIONALLICENCE 700 1- Amado Francisco Chemistry Department, University of Aveiro, Porto, Portugal aut NATIONALLICENCE 773 0- European Journal of Applied Physiology Springer-Verlag 97/3(2006-06-01), 340-346 1439-6319 97:3<340 2006 97 421 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer