caa a22 4500 467942218 CHVBK 20180406153016.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s00421-006-0220-y doi (NATIONALLICENCE)springer-10.1007/s00421-006-0220-y Muscle triacylglycerol and hormone-sensitive lipase activity in untrained and trained human muscles [Elektronische Daten] [Jørn Helge, Taus Biba, Henrik Galbo, Michael Gaster, Morten Donsmark] During exercise, triacylglycerol (TG) is recruited in skeletal muscles. We hypothesized that both muscle hormone-sensitive lipase (HSL) activity and TG recruitment would be higher in trained than in untrained subjects in response to prolonged exercise. Healthy male subjects (26±1years, body moss index 23.3±0.5kgm−2), either untrained (N=8, VO2max 3.8±0.2lmin−1) or trained (N=8, VO2max 5.1±0.1lmin−1), were studied. Before and after 3-h exercise (58±1% VO2max), a biopsy was taken. Muscle citrate synthase (32±2 vs. 47±6μmolg−1min−1 d.w.) and β-hydroxy-acyl-CoA-dehydrogenase (38±3 vs. 52±5μmolg−1min−1 d.w.) activities were lower in untrained than in trained subjects (p<0.05). Throughout the exercise, fat oxidation was higher in trained than in untrained subjects (p<0.05). Muscle HSL activity was similar at rest (0.72±0.08 and 0.74±0.03mUmg−1 protein) and after exercise (0.71±0.1 and 0.68±0.03mUmg−1 protein) in untrained and trained subjects. At rest, the chemically determined muscle TG content (37±8 and 26±5mmolg−1 d.w.) was similar (p>0.05), and after exercise it was unchanged in untrained and lower (p<0.05) in trained subjects (41±9 and 10±2mmolg(1 d.w.). Determined histochemically, TG was decreased (p<0.05) after exercise in type I and II fibres. Depletion of TG was not different between fibre types in untrained, but tended to be higher (p=0.07) in type I compared with type II fibres in trained muscles. In conclusion, HSL activity is similar in untrained and trained skeletal muscles both before and after prolonged exercise. However, the tendency to higher muscle TG recruitment during exercise in the trained subjects suggests a difference in the regulation of HSL or other lipases during exercise in trained compared with untrained subjects. Springer-Verlag, 2006 Muscle nationallicence Lipase nationallicence Substrate utilization nationallicence Exercise nationallicence Triacylglycerol nationallicence Helge Jørn Department of Medical Physiology, The Panum Institute, Copenhagen Muscle Research Centre, Building 12.4, Blegdamsvej 3, 2102, Copenhagen N, Denmark aut Biba Taus Departments of Pathology and Endocrinology, Odense University Hospital, Odense, Denmark aut Galbo Henrik Department of Medical Physiology, The Panum Institute, Copenhagen Muscle Research Centre, Building 12.4, Blegdamsvej 3, 2102, Copenhagen N, Denmark aut Gaster Michael Departments of Pathology and Endocrinology, Odense University Hospital, Odense, Denmark aut Donsmark Morten Department of Medical Physiology, The Panum Institute, Copenhagen Muscle Research Centre, Building 12.4, Blegdamsvej 3, 2102, Copenhagen N, Denmark aut European Journal of Applied Physiology Springer-Verlag 97/5(2006-07-01), 566-572 1439-6319 97:5<566 2006 97 421 https://doi.org/10.1007/s00421-006-0220-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00421-006-0220-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Helge Jørn Department of Medical Physiology, The Panum Institute, Copenhagen Muscle Research Centre, Building 12.4, Blegdamsvej 3, 2102, Copenhagen N, Denmark aut NATIONALLICENCE 700 1- Biba Taus Departments of Pathology and Endocrinology, Odense University Hospital, Odense, Denmark aut NATIONALLICENCE 700 1- Galbo Henrik Department of Medical Physiology, The Panum Institute, Copenhagen Muscle Research Centre, Building 12.4, Blegdamsvej 3, 2102, Copenhagen N, Denmark aut NATIONALLICENCE 700 1- Gaster Michael Departments of Pathology and Endocrinology, Odense University Hospital, Odense, Denmark aut NATIONALLICENCE 700 1- Donsmark Morten Department of Medical Physiology, The Panum Institute, Copenhagen Muscle Research Centre, Building 12.4, Blegdamsvej 3, 2102, Copenhagen N, Denmark aut NATIONALLICENCE 773 0- European Journal of Applied Physiology Springer-Verlag 97/5(2006-07-01), 566-572 1439-6319 97:5<566 2006 97 421 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer