caa a22 4500 467943834 CHVBK 20180323165659.0 cr unu---uuuuu 170328e19891001xx s 000 0 eng 10.1007/BF00553627 doi (NATIONALLICENCE)springer-10.1007/BF00553627 (NATIONALLICENCE)springer-10.1007/BF02396147 Differential expression of ribulose-1,5-bisphosphate carboxylase in reciprocal F1 hybrids of a C3 and a C4-like Flaveria species [Elektronische Daten] [Sarjit Johal, A. Holaday] Stable reciprocal hybrids betweenFlaveria pringlei (C3) andF. brownii (C4-like) have been produced by standard breeding techniques. There are no differences in the isoelectric focusing patterns of the catalytic subunits of the ribulose-1,5-bisphosphate carboxylase/oxygenase fromF. pringlei, F. brownii, or the reciprocal hybrids. The enzyme from both species also contains an identical noncatalytic subunit polypeptide. However, the carboxylase enzyme fromF. brownii contains another isomeric form of noncatalytic subunit polypeptide which is resolveable by isoelectric focusing. This isomeric form constitutes about 50% of the total noncatalytic subunits in this species. It comprises only about 10% of the total noncatalytic subunit population in the C3×C4 plants, but about 42% of the noncatalytic subunits in the reciprocal cross. The concentrations of the holoenzyme in the reciprocal hybrids are comparable to those of the respective maternal parent. We hypothesize that a differential inheritance of parental chloroplasts by the reciprocal hybrids may be associated with this apparent maternal influence on the expression of the noncatalytic polypeptides and the holoenzyme concentration. Plenum Publishing Corporation, 1989 ribulose-1,5-bisphosphate carboxylase/oxygenase nationallicence C3 nationallicence C4 nationallicence Flaveria nationallicence Johal Sarjit BP America, Research and Development, 44128, Cleveland, Ohio aut Holaday A. Department of Biological Sciences, Texas Tech University, 79409, Lubbock, Texas aut Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 27/9-10(1989-10-01), 497-505 0006-2928 27:9-10<497 1989 27 10528 https://doi.org/10.1007/BF00553627 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00553627 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Johal Sarjit BP America, Research and Development, 44128, Cleveland, Ohio aut NATIONALLICENCE 700 1- Holaday A. Department of Biological Sciences, Texas Tech University, 79409, Lubbock, Texas aut NATIONALLICENCE 773 0- Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 27/9-10(1989-10-01), 497-505 0006-2928 27:9-10<497 1989 27 10528 NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02396147 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer