caa a22 4500 467943842 CHVBK 20180405193735.0 cr unu---uuuuu 170328e20041001xx s 000 0 eng 10.1023/B:GROW.0000049419.22779.f5 doi (NATIONALLICENCE)springer-10.1023/B:GROW.0000049419.22779.f5 (NATIONALLICENCE)springer-10.1007/s10725-004-4113-y Differential induction of ornithine decarboxylase (ODC) gene family members in transgenic tobacco ( Nicotiana tabacum L. cv. Bright Yellow 2) cell suspensions by methyl-jasmonate treatment [Elektronische Daten] [Bingfang Xu, Moira Sheehan, Michael Timko] Two nuclear genes (NtODC-1 and NtODC-2) encoding ornithine decarboxylase (ODC, EC 4.1.1.17) were characterized from tobacco (Nicotiana tabacum L., and their differential induction by methyl-jasmonate (MeJA) was analyzed. ODC transcript levels increased in BY-2 cells following treatment with MeJA, with the highest levels of transcript observed approximately 5 h post-treatment. Analysis of transgenic BY-2 cell lines expressing a β-glucuronidase (GUS) reporter gene under the control of either the NtODC-1 or NtODC-2 gene promoter revealed that expression of the NtODC-1::GUS transgene was highly induced (8-fold) by treatment with MeJA, whereas the NtODC-2::GUS transgene showed little MeJA induced GUS expression. Removal of auxin (AUX, 2,4-D) from the cell growth media led to an increase in NtODC-1 and NtODC-2 transcript levels, whereas high levels of 2,4-D repressed overall ODC gene expression and suppressed the MeJA-induced expression of NtODC-1. Deletion analysis identified an upstream region (−924 to −1424 bp relative to the start site of transcription) of the NtODC-1 promoter that confers MeJA-responsiveness. No similar region was identified in the NtODC-2 promoter. Sequence analysis revealed that the NtODC-1 promoter fragment contains both G-box and TGACG-motif elements similar to those found in other MeJA-responsive genes. Our findings are discussed in terms of potential factors mediating different phytohormone-activated signal transduction pathways in the formation of nicotine and related compounds in tobacco. Kluwer Academic Publishers, 2004 Alkaloids nationallicence Gene expression nationallicence Jasmonic acid nationallicence Nicotiana tabacum L nationallicence Nicotine nationallicence Ornithine decarboxylase nationallicence Polyamine nationallicence Xu Bingfang Department of Biology, University of Virginia, 22903, Charlottesville, Virginia, USA aut Sheehan Moira Department of Biology, University of Virginia, 22903, Charlottesville, Virginia, USA aut Timko Michael Department of Biology, University of Virginia, 22903, Charlottesville, Virginia, USA aut Plant Growth Regulation Kluwer Academic Publishers 44/2(2004-10-01), 101-116 0167-6903 44:2<101 2004 44 10725 https://doi.org/10.1023/B:GROW.0000049419.22779.f5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/B:GROW.0000049419.22779.f5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu Bingfang Department of Biology, University of Virginia, 22903, Charlottesville, Virginia, USA aut NATIONALLICENCE 700 1- Sheehan Moira Department of Biology, University of Virginia, 22903, Charlottesville, Virginia, USA aut NATIONALLICENCE 700 1- Timko Michael Department of Biology, University of Virginia, 22903, Charlottesville, Virginia, USA aut NATIONALLICENCE 773 0- Plant Growth Regulation Kluwer Academic Publishers 44/2(2004-10-01), 101-116 0167-6903 44:2<101 2004 44 10725 NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-004-4113-y text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer