caa a22 4500 467943907 CHVBK 20180323165659.0 cr unu---uuuuu 170328e19891201xx s 000 0 eng 10.1007/BF02396057 doi (NATIONALLICENCE)springer-10.1007/BF02396057 (NATIONALLICENCE)springer-10.1007/BF00553986 Agarose gel electrophoresis in isozyme separation and visualization [Elektronische Daten] [Jay Hunt, Marshall Goren, Allan Tereba] Isozyme analysis of rodent-human somatic cell hybrids has been used frequently to detect specific human chromosomes. The majority of these isozyme systems employs starch gels, the use of which can be laborious when screening large numbers of cell lines. We describe the development of two procedures to detect the long arms of human chromosomes 1 and 2 in Chinese hamster-human cell hybrids by a rapid and reproducible method using 1-mm-thick agarose gels. Detection of human chromosome 1q was accomplished by screening for human fumarate hydratase activity, whose gene has been mapped to 1q42.1. Detection of chromosome 2q was performed by screening for the isozyme isocitrate dehydrogenase 1, which has been localized to 2q32-qter. These systems provide a basis for the further development of procedures for detecting chromosome-specific isozyme markers in agarose gels. Plenum Publishing Corporation, 1989 isozyme analyses nationallicence agarose gel electrophoresis nationallicence chromosome 1 nationallicence chromosome 2 nationallicence somatic cell hybrids nationallicence Hunt Jay Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, 38101, Memphis, Tennessee aut Goren Marshall Departments of Pathology and Laboratory Medicine, St. Jude Children's Research Hospital, 38101, Memphis, Tennessee aut Tereba Allan Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, 38101, Memphis, Tennessee aut Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 27/11-12(1989-12-01), 647-654 0006-2928 27:11-12<647 1989 27 10528 https://doi.org/10.1007/BF02396057 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02396057 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hunt Jay Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, 38101, Memphis, Tennessee aut NATIONALLICENCE 700 1- Goren Marshall Departments of Pathology and Laboratory Medicine, St. Jude Children's Research Hospital, 38101, Memphis, Tennessee aut NATIONALLICENCE 700 1- Tereba Allan Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, 38101, Memphis, Tennessee aut NATIONALLICENCE 773 0- Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 27/11-12(1989-12-01), 647-654 0006-2928 27:11-12<647 1989 27 10528 NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00553986 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer