caa a22 4500 467944431 CHVBK 20180405193735.0 cr unu---uuuuu 170328e20041101xx s 000 0 eng 10.1007/BF02637136 doi (NATIONALLICENCE)springer-10.1007/BF02637136 (NATIONALLICENCE)springer-10.1007/s00464-003-8928-9 The effect of timing of pneumoperitoneum on the inflammatory response [Elektronische Daten] [S. Bachman, E. Hanly, J. Nwanko, J. Lamb, A. Herring, M. Marohn, A. DeMaio, M. Talamini] Background: We examined the effects of an identical period of pneumoperitoneum applied at three different time points after lipopolysaccharide (LPS) challenge. Two different insufflation gases were also compared. Methods: Male rats (n=70) were injected intravenously with 1 mg/kg of LPS (time 0). The time relationship between a 1.5-h period of insufflation and initial LPS stimulation was the experimental variable. All rats were killed 6 h after injection. CO2 and helium insufflation were investigated. Ten control rats received LPS only. Serum interleukin-6 (IL-6) levels were determined by enzyme-linked immunosorbent assay (ELISA). Hepatic expression of α2-macroglobulin, β-fibrinogen, and metallothionein were measured by Northern blot analysis. Statistical analysis was performed using one-way analysis of variance (ANOVA). Results: Expression of α2-macroglobulin mRNA was lower in CO2 groups compared to the control group (p<0.05 at time 120 and 270). β-Fibrinogen message was diminished in CO2 0 and 120 groups compared to control. Serum levels of IL-6 and expression of metallothionein mRNA did not show significant differences between groups. Conclusions: These findings suggest that CO2 pneumoperitoneum downregulates the inflammatory response to LPS challenge. Start time of CO2 insufflation does not appear to alter hepatic expression of acute phase genes. The mechanism of α2-macroglobulin downregulation does not appear to be due to IL-6. Springer Science+Business Media, Inc, 2004 Laparoscopy nationallicence Pneumoperitoneum nationallicence Inflammatory response nationallicence Acute-phase proteins nationallicence Lipopolysaccharide nationallicence Endotoxemia nationallicence Bachman S. Center for Minimally Invasive Surgery, Department of Surgery, Johns Kopkins University School of Medicine, 21287, Baltimore, MD, USA aut Hanly E. Center for Minimally Invasive Surgery, Department of Surgery, Johns Kopkins University School of Medicine, 21287, Baltimore, MD, USA aut Nwanko J. Center for Minimally Invasive Surgery, Department of Surgery, Johns Kopkins University School of Medicine, 21287, Baltimore, MD, USA aut Lamb J. Center for Minimally Invasive Surgery, Department of Surgery, Johns Kopkins University School of Medicine, 21287, Baltimore, MD, USA aut Herring A. Center for Minimally Invasive Surgery, Department of Surgery, Johns Kopkins University School of Medicine, 21287, Baltimore, MD, USA aut Marohn M. Center for Minimally Invasive Surgery, Department of Surgery, Johns Kopkins University School of Medicine, 21287, Baltimore, MD, USA aut DeMaio A. Division of Pediatric Surgery, Johns Hopkins University School of Medicine, 21287, Baltimore, MD, USA aut Talamini M. Center for Minimally Invasive Surgery, Department of Surgery, Johns Kopkins University School of Medicine, 21287, Baltimore, MD, USA aut Surgical Endoscopy And Other Interventional Techniques Springer-Verlag 18/11(2004-11-01), 1640-1644 0930-2794 18:11<1640 2004 18 464 https://doi.org/10.1007/BF02637136 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02637136 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bachman S. Center for Minimally Invasive Surgery, Department of Surgery, Johns Kopkins University School of Medicine, 21287, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Hanly E. Center for Minimally Invasive Surgery, Department of Surgery, Johns Kopkins University School of Medicine, 21287, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Nwanko J. Center for Minimally Invasive Surgery, Department of Surgery, Johns Kopkins University School of Medicine, 21287, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Lamb J. Center for Minimally Invasive Surgery, Department of Surgery, Johns Kopkins University School of Medicine, 21287, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Herring A. Center for Minimally Invasive Surgery, Department of Surgery, Johns Kopkins University School of Medicine, 21287, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Marohn M. Center for Minimally Invasive Surgery, Department of Surgery, Johns Kopkins University School of Medicine, 21287, Baltimore, MD, USA aut NATIONALLICENCE 700 1- DeMaio A. Division of Pediatric Surgery, Johns Hopkins University School of Medicine, 21287, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Talamini M. Center for Minimally Invasive Surgery, Department of Surgery, Johns Kopkins University School of Medicine, 21287, Baltimore, MD, USA aut NATIONALLICENCE 773 0- Surgical Endoscopy And Other Interventional Techniques Springer-Verlag 18/11(2004-11-01), 1640-1644 0930-2794 18:11<1640 2004 18 464 NATIONALLICENCE 856 40 https://doi.org/10.1007/s00464-003-8928-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bachman S. Center for Minimally Invasive Surgery, Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA aut NATIONALLICENCE 700 1- Hanly E. Center for Minimally Invasive Surgery, Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA aut NATIONALLICENCE 700 1- Nwanko J. Center for Minimally Invasive Surgery, Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA aut NATIONALLICENCE 700 1- Lamb J. Center for Minimally Invasive Surgery, Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA aut NATIONALLICENCE 700 1- Herring A. Center for Minimally Invasive Surgery, Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA aut NATIONALLICENCE 700 1- Marohn M. Center for Minimally Invasive Surgery, Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA aut NATIONALLICENCE 700 1- De Maio A. Division of Pediatric Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA aut NATIONALLICENCE 700 1- Talamini M. Center for Minimally Invasive Surgery, Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA aut NATIONALLICENCE 773 0- Surgical Endoscopy And Other Interventional Techniques Springer-Verlag; www.springer-ny.com 18/11(2004-11-01), 1640-1644 0930-2794 18:11<1640 2004 18 464 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 46751206X BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer