caa a22 4500 467944490 CHVBK 20180323165659.0 cr unu---uuuuu 170328e19881201xx s 000 0 eng 10.1007/BF00553875 doi (NATIONALLICENCE)springer-10.1007/BF00553875 (NATIONALLICENCE)springer-10.1007/BF02395522 Effects of deletions in mouse chromosome 7 on expression of genes encoding the urea-cycle enzymes and phosphoenolpyruvate carboxykinase (GTP) in liver, kidney, and intestine [Elektronische Daten] [Sidney Morris Jr., Carole Moncman, Diane Kepka, Vicki Nebes, Warren Diven, George Dizikes, Stephen Cederbaum, Donald DeFranco] Chromosomal deletions at and around the albino locus on chromosome 7 of the mouse affect the enzyme activities and steady-state levels of mRNAs for five urea-cycle enzymes in liver. In newbornc 3H homozygotes, activities of these enzymes were 43-62% of normal, while corresponding mRNA levels were 14-29% of normal.c 14CoS deletion homozygotes expressed mRNA levels for these enzymes which were 32-48% of normal. However, transcription rates of these genes in hepatic nuclei ofc 3H/c3H mice were reduced only to 57-84% of normal. Since effects of the deletions had previously been noted in the kidney, mRNA levels for three enzymes expressed also in the kidney were examined. Mice homozygous for thec 3H deletion, shown previously to have drastically reduced mRNA levels for phosphoenolpyruvate carboxykinase in the liver, expressed the same deficiency in the kidney, while mRNA levels for argininosuccinate synthetase and argininosuccinate lyase were reduced in the liver but remained unaffected in the kidney. However, mRNA levels for phosphoenolpyruvate carboxykinase, carbamyl phosphate synthetase I, and ornithine transcarbamylase were unaffected in the intestine ofc 3H homozygotes. The results suggest that a regulatory factor(s) encoded in the DNA encompassed by the deletion is involved in the normal developmental maturation of hepatocytes and certain cells in the kidney. Plenum Publishing Corporation, 1988 urea-cycle enzymes nationallicence phosphoenolpyruvate carboxykinase nationallicence hepatocyte development nationallicence tissue-specific gene expression nationallicence mutant mice nationallicence Morris Jr. Sidney Departments of Microbiology, Biochemistry, and Molecular Biology, University of Pittsburgh, 15261, Pittsburgh, Pennsylvania aut Moncman Carole Departments of Microbiology, Biochemistry, and Molecular Biology, University of Pittsburgh, 15261, Pittsburgh, Pennsylvania aut Kepka Diane Departments of Microbiology, Biochemistry, and Molecular Biology, University of Pittsburgh, 15261, Pittsburgh, Pennsylvania aut Nebes Vicki Departments of Microbiology, Biochemistry, and Molecular Biology, University of Pittsburgh, 15261, Pittsburgh, Pennsylvania aut Diven Warren Departments of Microbiology, Biochemistry, and Molecular Biology, University of Pittsburgh, 15261, Pittsburgh, Pennsylvania aut Dizikes George Departments of Psychiatry and Pediatrics and the Mental Retardation Research Center, UCLA Center for the Health Sciences, 90024, Los Angeles, California aut Cederbaum Stephen Departments of Psychiatry and Pediatrics and the Mental Retardation Research Center, UCLA Center for the Health Sciences, 90024, Los Angeles, California aut DeFranco Donald Department of Biological Sciences, University of Pittsburgh, 15261, Pittsburgh, Pennsylvania aut Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 26/11-12(1988-12-01), 769-781 0006-2928 26:11-12<769 1988 26 10528 https://doi.org/10.1007/BF00553875 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00553875 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Morris Jr Sidney Departments of Microbiology, Biochemistry, and Molecular Biology, University of Pittsburgh, 15261, Pittsburgh, Pennsylvania aut NATIONALLICENCE 700 1- Moncman Carole Departments of Microbiology, Biochemistry, and Molecular Biology, University of Pittsburgh, 15261, Pittsburgh, Pennsylvania aut NATIONALLICENCE 700 1- Kepka Diane Departments of Microbiology, Biochemistry, and Molecular Biology, University of Pittsburgh, 15261, Pittsburgh, Pennsylvania aut NATIONALLICENCE 700 1- Nebes Vicki Departments of Microbiology, Biochemistry, and Molecular Biology, University of Pittsburgh, 15261, Pittsburgh, Pennsylvania aut NATIONALLICENCE 700 1- Diven Warren Departments of Microbiology, Biochemistry, and Molecular Biology, University of Pittsburgh, 15261, Pittsburgh, Pennsylvania aut NATIONALLICENCE 700 1- Dizikes George Departments of Psychiatry and Pediatrics and the Mental Retardation Research Center, UCLA Center for the Health Sciences, 90024, Los Angeles, California aut NATIONALLICENCE 700 1- Cederbaum Stephen Departments of Psychiatry and Pediatrics and the Mental Retardation Research Center, UCLA Center for the Health Sciences, 90024, Los Angeles, California aut NATIONALLICENCE 700 1- DeFranco Donald Department of Biological Sciences, University of Pittsburgh, 15261, Pittsburgh, Pennsylvania aut NATIONALLICENCE 773 0- Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 26/11-12(1988-12-01), 769-781 0006-2928 26:11-12<769 1988 26 10528 NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02395522 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer